ENSG00000143314

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000361531 ENSG00000143314 HEK293_DMSO_6hA HEK293_OSMI2_6hA MRPL24 protein_coding protein_coding 156.7954 127.0188 189.6991 12.58968 38.06508 0.5786339 77.750506 59.28795 95.598917 7.278700 19.551547 0.6891630 0.4854833 0.46460000 0.50263333 0.03803333 0.5910462 0.0123776 FALSE  
ENST00000368211 ENSG00000143314 HEK293_DMSO_6hA HEK293_OSMI2_6hA MRPL24 protein_coding protein_coding 156.7954 127.0188 189.6991 12.58968 38.06508 0.5786339 66.207568 49.36051 83.905113 4.223603 18.927137 0.7652811 0.4188500 0.39003333 0.43846667 0.04843333 0.3936548 0.0123776 FALSE  
ENST00000478899 ENSG00000143314 HEK293_DMSO_6hA HEK293_OSMI2_6hA MRPL24 protein_coding processed_transcript 156.7954 127.0188 189.6991 12.58968 38.06508 0.5786339 6.256943 10.58741 2.763449 2.031343 1.220046 -1.9339580 0.0456250 0.08213333 0.01606667 -0.06606667 0.0123776 0.0123776 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000143314 E001 13.70194 4.252815e-03 0.182137583 0.58211456 1 156737303 156737304 2 - 1.222 1.097 -0.450
ENSG00000143314 E002 1147.00109 8.064222e-04 0.438568673 0.79687188 1 156737305 156737529 225 - 3.050 3.064 0.048
ENSG00000143314 E003 674.27891 3.518684e-04 0.052629495 0.35173076 1 156737530 156737534 5 - 2.810 2.842 0.106
ENSG00000143314 E004 1446.64143 2.037755e-04 0.381983373 0.76153342 1 156737646 156737776 131 - 3.151 3.161 0.035
ENSG00000143314 E005 1089.19039 2.082967e-04 0.648223144 0.89579760 1 156738031 156738117 87 - 3.035 3.029 -0.019
ENSG00000143314 E006 608.99304 3.704335e-04 0.911149062 0.97871640 1 156738118 156738134 17 - 2.780 2.781 0.003
ENSG00000143314 E007 13.71730 6.268005e-02 0.001339845 0.06400239 1 156738135 156738227 93 - 0.882 1.361 1.730
ENSG00000143314 E008 889.75071 7.552905e-05 0.377560303 0.75845550 1 156738343 156738417 75 - 2.949 2.938 -0.037
ENSG00000143314 E009 693.61844 1.058910e-04 0.178622727 0.57757669 1 156738418 156738438 21 - 2.845 2.826 -0.063
ENSG00000143314 E010 1401.20248 1.040150e-03 0.477900584 0.81817966 1 156738522 156738764 243 - 3.147 3.139 -0.027
ENSG00000143314 E011 26.47871 8.508607e-02 0.061178527 0.37464274 1 156740379 156740562 184 - 1.287 1.568 0.972
ENSG00000143314 E012 34.54986 1.817480e-02 0.063710101 0.38100751 1 156740931 156741011 81 - 1.466 1.630 0.559
ENSG00000143314 E013 358.08965 2.196045e-03 0.537925185 0.84881917 1 156741012 156741052 41 - 2.559 2.545 -0.048
ENSG00000143314 E014 466.55946 8.516863e-04 0.050577687 0.34581357 1 156741053 156741131 79 - 2.685 2.644 -0.139
ENSG00000143314 E015 15.86966 1.708856e-02 0.134807245 0.51756329 1 156741180 156741590 411 - 1.149 1.298 0.530