ENSG00000143303

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368216 ENSG00000143303 HEK293_DMSO_6hA HEK293_OSMI2_6hA METTL25B protein_coding protein_coding 29.16255 42.02007 42.66742 3.219227 1.861432 0.02205125 10.365571 11.7434062 16.343218 1.1870664 1.3720324 0.47649560 0.36045000 0.27966667 0.38486667 0.105200000 0.255430592 0.002445171 FALSE  
ENST00000368218 ENSG00000143303 HEK293_DMSO_6hA HEK293_OSMI2_6hA METTL25B protein_coding protein_coding 29.16255 42.02007 42.66742 3.219227 1.861432 0.02205125 1.263921 0.4277479 3.423159 0.4277479 1.1931771 2.97136443 0.04414583 0.00910000 0.08133333 0.072233333 0.167667520 0.002445171 FALSE  
ENST00000484742 ENSG00000143303 HEK293_DMSO_6hA HEK293_OSMI2_6hA METTL25B protein_coding protein_coding 29.16255 42.02007 42.66742 3.219227 1.861432 0.02205125 3.097556 6.5521213 2.389212 0.9109302 2.3892117 -1.45160179 0.11162500 0.15436667 0.05423333 -0.100133333 0.320081422 0.002445171 FALSE  
ENST00000497515 ENSG00000143303 HEK293_DMSO_6hA HEK293_OSMI2_6hA METTL25B protein_coding processed_transcript 29.16255 42.02007 42.66742 3.219227 1.861432 0.02205125 2.791215 7.3144785 3.342407 0.9388488 0.6176488 -1.12752879 0.09054167 0.17296667 0.07800000 -0.094966667 0.002445171 0.002445171    
ENST00000517871 ENSG00000143303 HEK293_DMSO_6hA HEK293_OSMI2_6hA METTL25B protein_coding retained_intron 29.16255 42.02007 42.66742 3.219227 1.861432 0.02205125 1.451792 2.5755526 1.864882 0.5666681 0.8859754 -0.46367295 0.05326667 0.06293333 0.04343333 -0.019500000 0.743953650 0.002445171 FALSE  
ENST00000524343 ENSG00000143303 HEK293_DMSO_6hA HEK293_OSMI2_6hA METTL25B protein_coding protein_coding 29.16255 42.02007 42.66742 3.219227 1.861432 0.02205125 1.872832 2.0479843 2.489673 0.5075265 0.6099177 0.28050729 0.06835417 0.05120000 0.05830000 0.007100000 0.868548583 0.002445171    
MSTRG.2378.16 ENSG00000143303 HEK293_DMSO_6hA HEK293_OSMI2_6hA METTL25B protein_coding   29.16255 42.02007 42.66742 3.219227 1.861432 0.02205125 1.497543 0.5751228 3.462241 0.5751228 1.7931300 2.56905559 0.04944167 0.01333333 0.08200000 0.068666667 0.689068366 0.002445171 FALSE  
MSTRG.2378.4 ENSG00000143303 HEK293_DMSO_6hA HEK293_OSMI2_6hA METTL25B protein_coding   29.16255 42.02007 42.66742 3.219227 1.861432 0.02205125 2.391583 3.6428426 3.799509 0.2936452 0.2671010 0.06058532 0.07535417 0.08663333 0.08886667 0.002233333 0.968064775 0.002445171 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000143303 E001 0.2937266 0.0274424043 0.247833350   1 156728442 156728468 27 + 0.199 0.000 -9.472
ENSG00000143303 E002 21.4847650 0.0013547151 0.146514808 0.53437170 1 156728469 156728534 66 + 1.396 1.293 -0.356
ENSG00000143303 E003 21.8865687 0.0013702185 0.096137206 0.45020065 1 156728535 156728559 25 + 1.410 1.293 -0.407
ENSG00000143303 E004 251.2641096 0.0001386749 0.001051597 0.05585227 1 156728560 156728948 389 + 2.431 2.364 -0.221
ENSG00000143303 E005 141.7537112 0.0002408071 0.115625029 0.48558947 1 156728949 156728959 11 + 2.173 2.133 -0.136
ENSG00000143303 E006 206.6142760 0.0002011739 0.023845840 0.25377417 1 156728960 156729030 71 + 2.340 2.290 -0.167
ENSG00000143303 E007 132.8086808 0.0013719169 0.168544800 0.56538400 1 156729031 156729044 14 + 2.147 2.103 -0.148
ENSG00000143303 E008 220.5441872 0.0001998126 0.016215760 0.21599161 1 156729045 156729127 83 + 2.369 2.317 -0.172
ENSG00000143303 E009 137.0683542 0.0002162252 0.075073647 0.40712025 1 156729128 156729162 35 + 2.160 2.112 -0.159
ENSG00000143303 E010 138.2089286 0.0002481466 0.498180665 0.82853907 1 156729163 156729177 15 + 2.148 2.134 -0.048
ENSG00000143303 E011 167.0199859 0.0002880208 0.393995459 0.76939680 1 156729178 156729215 38 + 2.231 2.213 -0.058
ENSG00000143303 E012 33.8334013 0.0344865322 0.666189758 0.90277063 1 156729448 156729515 68 + 1.523 1.552 0.099
ENSG00000143303 E013 36.3225267 0.0405632117 0.514366091 0.83729763 1 156729516 156729562 47 + 1.540 1.598 0.201
ENSG00000143303 E014 138.7336587 0.0002457001 0.991854462 0.99803910 1 156731991 156732024 34 + 2.140 2.146 0.019
ENSG00000143303 E015 199.6601652 0.0001831971 0.949381245 0.98815778 1 156732025 156732115 91 + 2.297 2.302 0.015
ENSG00000143303 E016 81.6093351 0.0079353068 0.021400354 0.24249394 1 156732116 156732251 136 + 1.840 1.980 0.472
ENSG00000143303 E017 53.5144627 0.0130531145 0.098781013 0.45494450 1 156732252 156732280 29 + 1.668 1.791 0.418
ENSG00000143303 E018 87.5968323 0.0004238498 0.977613784 0.99472794 1 156732281 156732286 6 + 1.943 1.950 0.023
ENSG00000143303 E019 163.0228674 0.0002333965 0.990599063 0.99767545 1 156732287 156732365 79 + 2.208 2.215 0.021
ENSG00000143303 E020 128.5483378 0.0002887820 0.256414325 0.66214994 1 156732366 156732384 19 + 2.091 2.131 0.137
ENSG00000143303 E021 222.2533890 0.0002154238 0.263217497 0.66821073 1 156732385 156732473 89 + 2.330 2.362 0.106
ENSG00000143303 E022 201.1779354 0.0002125905 0.277154944 0.68070299 1 156732985 156733047 63 + 2.286 2.319 0.110
ENSG00000143303 E023 216.5996147 0.0001621318 0.516904350 0.83861076 1 156733377 156733471 95 + 2.339 2.329 -0.032
ENSG00000143303 E024 160.6665278 0.0002488059 0.825635808 0.95572386 1 156733472 156733520 49 + 2.205 2.205 0.000
ENSG00000143303 E025 21.3866079 0.1346380386 0.099454454 0.45620756 1 156733521 156733625 105 + 1.211 1.452 0.842
ENSG00000143303 E026 13.3437490 0.0608275493 0.049019241 0.34161022 1 156733626 156733649 24 + 0.991 1.270 1.003
ENSG00000143303 E027 135.3267192 0.0002676573 0.283801435 0.68653672 1 156734009 156734052 44 + 2.113 2.151 0.128
ENSG00000143303 E028 304.4086303 0.0002148861 0.422997783 0.78788894 1 156734053 156734459 407 + 2.487 2.476 -0.034
ENSG00000143303 E029 88.8885892 0.0003369279 0.004683299 0.12288695 1 156734460 156734493 34 + 1.996 1.896 -0.336
ENSG00000143303 E030 26.0281456 0.0449846294 0.001371032 0.06480355 1 156735622 156735724 103 + 1.199 1.583 1.331
ENSG00000143303 E031 288.4764998 0.0002107179 0.615028644 0.88225452 1 156735725 156735909 185 + 2.460 2.456 -0.013
ENSG00000143303 E032 24.2666599 0.0170306823 0.020192982 0.23687942 1 156736158 156736584 427 + 1.266 1.495 0.794
ENSG00000143303 E033 29.2089058 0.0010508350 0.508113811 0.83384243 1 156736619 156736631 13 + 1.499 1.462 -0.126
ENSG00000143303 E034 192.8315566 0.0009620788 0.070372771 0.39646587 1 156736632 156736680 49 + 2.253 2.313 0.201
ENSG00000143303 E035 240.7832599 0.0012733166 0.001030609 0.05516191 1 156736681 156736960 280 + 2.327 2.428 0.336