ENSG00000136527

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000259043 ENSG00000136527 HEK293_DMSO_6hA HEK293_OSMI2_6hA TRA2B protein_coding protein_coding 186.2608 112.4264 72.71138 18.23014 5.469579 -0.6286572 32.317600 18.782768 13.704865 3.865094 2.4801656 -0.4544372 0.18082500 0.16596667 0.18606667 0.020100000 0.76932462 0.03912837 FALSE FALSE
ENST00000382191 ENSG00000136527 HEK293_DMSO_6hA HEK293_OSMI2_6hA TRA2B protein_coding protein_coding 186.2608 112.4264 72.71138 18.23014 5.469579 -0.6286572 51.254498 22.974110 15.151015 3.955348 1.4899682 -0.6002705 0.25371667 0.20446667 0.20993333 0.005466667 0.96889269 0.03912837   TRUE
ENST00000414862 ENSG00000136527 HEK293_DMSO_6hA HEK293_OSMI2_6hA TRA2B protein_coding protein_coding 186.2608 112.4264 72.71138 18.23014 5.469579 -0.6286572 9.689225 10.614491 4.396427 2.707484 0.1676843 -1.2697125 0.05995833 0.09486667 0.06116667 -0.033700000 0.39625507 0.03912837 FALSE TRUE
ENST00000456380 ENSG00000136527 HEK293_DMSO_6hA HEK293_OSMI2_6hA TRA2B protein_coding nonsense_mediated_decay 186.2608 112.4264 72.71138 18.23014 5.469579 -0.6286572 45.229504 30.404590 19.870256 6.016691 1.6369906 -0.6134272 0.24969167 0.26950000 0.27300000 0.003500000 0.97585835 0.03912837 FALSE TRUE
ENST00000485530 ENSG00000136527 HEK293_DMSO_6hA HEK293_OSMI2_6hA TRA2B protein_coding retained_intron 186.2608 112.4264 72.71138 18.23014 5.469579 -0.6286572 8.005775 12.290359 2.732943 1.402053 2.0677281 -2.1649041 0.04448333 0.11233333 0.04263333 -0.069700000 0.64504620 0.03912837 FALSE FALSE
MSTRG.24221.3 ENSG00000136527 HEK293_DMSO_6hA HEK293_OSMI2_6hA TRA2B protein_coding   186.2608 112.4264 72.71138 18.23014 5.469579 -0.6286572 30.498987 6.584431 12.585840 1.843585 3.6009144 0.9336273 0.16032083 0.05750000 0.16713333 0.109633333 0.03912837 0.03912837 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000136527 E001 17.615715 0.0065313842 7.552400e-01 0.934327379 3 185914558 185916743 2186 - 1.284 1.242 -0.147
ENSG00000136527 E002 4.551881 0.0511317200 9.596784e-01 0.990717692 3 185916744 185916748 5 - 0.729 0.742 0.051
ENSG00000136527 E003 332.222726 0.0329605535 1.190417e-02 0.188605855 3 185916749 185917154 406 - 2.643 2.403 -0.798
ENSG00000136527 E004 313.236414 0.0331092035 1.105607e-02 0.181953516 3 185917155 185917327 173 - 2.620 2.375 -0.818
ENSG00000136527 E005 130.149706 0.0221486446 9.522085e-03 0.169525046 3 185917328 185917328 1 - 2.229 2.009 -0.737
ENSG00000136527 E006 129.786204 0.0205114574 7.478409e-03 0.152153378 3 185917329 185917329 1 - 2.227 2.008 -0.734
ENSG00000136527 E007 1127.901382 0.0016890271 4.194871e-01 0.785750051 3 185917330 185917714 385 - 3.054 3.024 -0.102
ENSG00000136527 E008 452.776677 0.0015877279 1.711090e-01 0.568461866 3 185917715 185917725 11 - 2.624 2.645 0.070
ENSG00000136527 E009 634.119592 0.0009746681 8.715966e-02 0.432355226 3 185918365 185918435 71 - 2.770 2.791 0.070
ENSG00000136527 E010 340.203061 0.0001939615 2.885204e-02 0.275058443 3 185918436 185918438 3 - 2.493 2.525 0.105
ENSG00000136527 E011 490.690239 0.0007528505 9.157715e-02 0.441239630 3 185919437 185919496 60 - 2.660 2.682 0.073
ENSG00000136527 E012 705.799046 0.0006653050 1.917994e-01 0.593922612 3 185921104 185921187 84 - 2.824 2.835 0.034
ENSG00000136527 E013 619.115871 0.0006676467 5.881627e-02 0.368488155 3 185922011 185922042 32 - 2.759 2.782 0.076
ENSG00000136527 E014 772.329755 0.0012837106 1.114685e-02 0.182718257 3 185922043 185922126 84 - 2.844 2.885 0.136
ENSG00000136527 E015 51.091074 0.0644205338 8.698400e-02 0.431945807 3 185922127 185923795 1669 - 1.568 1.757 0.643
ENSG00000136527 E016 921.535881 0.0004562031 1.750817e-01 0.572909602 3 185923796 185923984 189 - 2.942 2.950 0.026
ENSG00000136527 E017 4.142276 0.0054451501 7.903405e-02 0.415523507 3 185923985 185924008 24 - 0.529 0.783 1.090
ENSG00000136527 E018 10.311115 0.0335650033 4.637921e-02 0.334035908 3 185924009 185924804 796 - 0.864 1.118 0.939
ENSG00000136527 E019 3.738708 0.0060259232 1.803551e-01 0.579642634 3 185924805 185925084 280 - 0.529 0.725 0.855
ENSG00000136527 E020 2.772327 0.0407680129 4.213680e-01 0.786970986 3 185925085 185925229 145 - 0.473 0.608 0.629
ENSG00000136527 E021 2.098782 0.0091472082 2.113738e-01 0.615872887 3 185925230 185925272 43 - 0.340 0.548 1.090
ENSG00000136527 E022 5.629822 0.0526661767 1.039334e-01 0.464549099 3 185925273 185925463 191 - 0.624 0.879 1.033
ENSG00000136527 E023 806.013860 0.0004481273 2.828810e-01 0.685731606 3 185925464 185925626 163 - 2.907 2.878 -0.097
ENSG00000136527 E024 596.975194 0.0005707003 5.600153e-01 0.858039816 3 185926601 185926726 126 - 2.773 2.749 -0.077
ENSG00000136527 E025 233.872191 0.0001470075 8.252560e-01 0.955713247 3 185926727 185926734 8 - 2.364 2.346 -0.060
ENSG00000136527 E026 230.071706 0.0515371057 6.371684e-03 0.141058409 3 185926735 185929073 2339 - 2.142 2.430 0.962
ENSG00000136527 E027 8.686713 0.0037062263 3.668456e-05 0.007191876 3 185931515 185931576 62 - 0.577 1.102 2.067
ENSG00000136527 E028 37.737757 0.0321961916 5.725246e-01 0.863278400 3 185931577 185931852 276 - 1.531 1.595 0.218
ENSG00000136527 E029 7.030981 0.1975664960 5.285003e-01 0.844263003 3 185934429 185934505 77 - 0.805 0.928 0.472
ENSG00000136527 E030 38.545907 0.1243450377 7.553879e-01 0.934409951 3 185934506 185936237 1732 - 1.566 1.589 0.078
ENSG00000136527 E031 19.507138 0.1658580705 7.996038e-01 0.948180253 3 185936238 185937420 1183 - 1.280 1.302 0.079
ENSG00000136527 E032 428.887229 0.0005741112 3.123743e-01 0.710135935 3 185937825 185938103 279 - 2.638 2.603 -0.116