ENSG00000135763

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258243 ENSG00000135763 HEK293_DMSO_6hA HEK293_OSMI2_6hA URB2 protein_coding protein_coding 26.79841 26.55083 28.06722 1.014555 2.28566 0.08009986 23.850418 22.638107 26.5925051 1.2273616 1.9578411 0.2321716 0.8966375 0.85200000 0.9486 0.09660000 0.0005130999 0.0005130999 FALSE TRUE
MSTRG.3237.3 ENSG00000135763 HEK293_DMSO_6hA HEK293_OSMI2_6hA URB2 protein_coding   26.79841 26.55083 28.06722 1.014555 2.28566 0.08009986 1.954131 2.396663 0.8032273 0.6717585 0.4604609 -1.5653035 0.0648625 0.08966667 0.0264 -0.06326667 0.2578217306 0.0005130999 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000135763 E001 96.14575 2.391467e-03 3.882938e-01 0.76600884 1 229626247 229626356 110 + 2.007 1.964 -0.143
ENSG00000135763 E002 156.22494 7.697190e-04 7.908553e-02 0.41566809 1 229627621 229627759 139 + 2.222 2.164 -0.196
ENSG00000135763 E003 206.61144 8.397531e-03 3.958781e-01 0.77050744 1 229632269 229632445 177 + 2.344 2.287 -0.190
ENSG00000135763 E004 1039.93110 2.981558e-03 1.785134e-01 0.57744582 1 229634917 229636416 1500 + 3.037 2.994 -0.143
ENSG00000135763 E005 174.17378 4.887203e-04 5.190097e-03 0.12901977 1 229636417 229636519 103 + 2.275 2.193 -0.275
ENSG00000135763 E006 357.96247 1.060959e-03 6.974727e-03 0.14726958 1 229636520 229636800 281 + 2.585 2.515 -0.231
ENSG00000135763 E007 1304.11190 9.613257e-05 7.540045e-01 0.93378946 1 229636801 229638181 1381 + 3.112 3.105 -0.024
ENSG00000135763 E008 227.86721 2.126406e-03 2.357255e-01 0.64057244 1 229638182 229638247 66 + 2.335 2.370 0.117
ENSG00000135763 E009 339.29781 1.675382e-03 1.411150e-01 0.52685578 1 229643533 229643693 161 + 2.506 2.542 0.121
ENSG00000135763 E010 251.62873 9.501101e-04 3.665714e-01 0.75131949 1 229645859 229645969 111 + 2.385 2.405 0.066
ENSG00000135763 E011 412.46612 1.236050e-04 2.376716e-01 0.64286856 1 229647510 229647752 243 + 2.600 2.616 0.055
ENSG00000135763 E012 11.96734 5.246360e-02 2.976989e-02 0.27857836 1 229650847 229651234 388 + 0.933 1.231 1.084
ENSG00000135763 E013 214.57175 2.196997e-04 2.575399e-01 0.66326009 1 229651235 229651322 88 + 2.313 2.337 0.079
ENSG00000135763 E014 307.72581 5.339333e-04 5.737116e-01 0.86380678 1 229654249 229654388 140 + 2.477 2.485 0.028
ENSG00000135763 E015 922.76426 2.409441e-04 6.662149e-05 0.01077052 1 229659100 229660200 1101 + 2.934 2.978 0.148