ENSG00000135632

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000389501 ENSG00000135632 HEK293_DMSO_6hA HEK293_OSMI2_6hA SMYD5 protein_coding protein_coding 43.50134 37.344 59.32865 2.969276 9.399362 0.6677093 38.211322 31.155014 54.389692 2.8117085 10.3925497 0.8036711 0.85322500 0.83273333 0.90480000 0.07206667 0.28507491 0.04971541 FALSE TRUE
ENST00000477038 ENSG00000135632 HEK293_DMSO_6hA HEK293_OSMI2_6hA SMYD5 protein_coding retained_intron 43.50134 37.344 59.32865 2.969276 9.399362 0.6677093 2.170742 2.994902 1.345589 0.2621778 0.2969435 -1.1483983 0.06457917 0.08013333 0.02606667 -0.05406667 0.04971541 0.04971541 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000135632 E001 0.1767706 0.0520335430 7.040313e-01   2 73214222 73214237 16 + 0.099 0.000 -8.792
ENSG00000135632 E002 0.4018037 0.0233044605 1.758935e-01   2 73214238 73214244 7 + 0.247 0.000 -12.732
ENSG00000135632 E003 33.0646204 0.0032709505 6.482632e-02 0.383924987 2 73214245 73214266 22 + 1.570 1.449 -0.413
ENSG00000135632 E004 116.9257328 0.0075263778 3.487562e-01 0.738459726 2 73214267 73214362 96 + 2.087 2.040 -0.159
ENSG00000135632 E005 3.0752752 0.1928212046 7.514828e-01 0.932988688 2 73214567 73214589 23 + 0.570 0.619 0.216
ENSG00000135632 E006 6.8499375 0.1866419074 4.131431e-01 0.782403445 2 73214590 73214705 116 + 0.804 0.979 0.671
ENSG00000135632 E007 6.5801580 0.2213771384 5.142130e-01 0.837212336 2 73214706 73214749 44 + 0.810 0.942 0.508
ENSG00000135632 E008 219.2490696 0.0026414098 9.933414e-01 0.998382251 2 73218861 73218969 109 + 2.338 2.339 0.004
ENSG00000135632 E009 5.5712404 0.2079291198 2.243072e-01 0.629208222 2 73219917 73219951 35 + 0.683 0.929 0.972
ENSG00000135632 E010 12.8266768 0.0900066474 8.825556e-01 0.971859231 2 73219952 73220050 99 + 1.125 1.147 0.080
ENSG00000135632 E011 155.3232222 0.0002494013 7.617858e-01 0.936406085 2 73220051 73220069 19 + 2.189 2.197 0.027
ENSG00000135632 E012 302.1582801 0.0008888345 8.793041e-01 0.970978875 2 73220070 73220190 121 + 2.477 2.482 0.017
ENSG00000135632 E013 328.4072095 0.0001499819 7.502151e-01 0.932579481 2 73220661 73220782 122 + 2.518 2.512 -0.022
ENSG00000135632 E014 174.1847782 0.0001979049 1.526317e-01 0.542863647 2 73221165 73221175 11 + 2.221 2.259 0.128
ENSG00000135632 E015 259.7858627 0.0001797599 9.954000e-01 0.998761747 2 73221176 73221234 59 + 2.410 2.410 0.000
ENSG00000135632 E016 384.4560463 0.0022906008 9.593590e-01 0.990654048 2 73221826 73221930 105 + 2.578 2.581 0.010
ENSG00000135632 E017 288.5589098 0.0003244445 7.576144e-01 0.935075834 2 73222755 73222817 63 + 2.452 2.460 0.025
ENSG00000135632 E018 317.0836852 0.0001272893 8.965610e-01 0.975336960 2 73223036 73223106 71 + 2.499 2.496 -0.009
ENSG00000135632 E019 8.0186000 0.0082280648 4.141585e-05 0.007834451 2 73223107 73223167 61 + 0.662 1.157 1.897
ENSG00000135632 E020 377.0269921 0.0001216356 9.039657e-01 0.977239216 2 73223426 73223532 107 + 2.575 2.573 -0.008
ENSG00000135632 E021 261.3533706 0.0001842733 4.367385e-01 0.795669301 2 73223947 73224003 57 + 2.424 2.406 -0.059
ENSG00000135632 E022 326.0388686 0.0008950243 2.213855e-01 0.625862821 2 73224866 73224960 95 + 2.522 2.495 -0.090
ENSG00000135632 E023 9.3807251 0.1055845920 1.288722e-01 0.508507565 2 73225222 73225630 409 + 0.855 1.148 1.083
ENSG00000135632 E024 279.7998132 0.0009469623 1.915099e-01 0.593646812 2 73225631 73225701 71 + 2.458 2.427 -0.102
ENSG00000135632 E025 1836.9662796 0.0012928799 6.345228e-01 0.889967861 2 73225796 73227221 1426 + 3.252 3.269 0.057