ENSG00000135476

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000257934 ENSG00000135476 HEK293_DMSO_6hA HEK293_OSMI2_6hA ESPL1 protein_coding protein_coding 33.4473 37.04769 51.2851 3.279847 5.074952 0.4690482 17.070155 14.892534 28.729185 2.0657172 2.6758718 0.9474615 0.5235292 0.39910000 0.56150000 0.16240000 0.003817998 0.003817998 FALSE TRUE
ENST00000549154 ENSG00000135476 HEK293_DMSO_6hA HEK293_OSMI2_6hA ESPL1 protein_coding retained_intron 33.4473 37.04769 51.2851 3.279847 5.074952 0.4690482 3.447937 5.974352 4.476754 0.2490378 1.0873443 -0.4155228 0.1056000 0.16353333 0.09226667 -0.07126667 0.299453185 0.003817998 FALSE TRUE
MSTRG.7351.1 ENSG00000135476 HEK293_DMSO_6hA HEK293_OSMI2_6hA ESPL1 protein_coding   33.4473 37.04769 51.2851 3.279847 5.074952 0.4690482 3.348198 3.209394 5.145932 0.5823000 0.2960246 0.6794443 0.1035458 0.08973333 0.10123333 0.01150000 0.783034533 0.003817998 TRUE TRUE
MSTRG.7351.4 ENSG00000135476 HEK293_DMSO_6hA HEK293_OSMI2_6hA ESPL1 protein_coding   33.4473 37.04769 51.2851 3.279847 5.074952 0.4690482 4.262458 5.803081 6.519961 1.3653565 2.1658316 0.1677714 0.1078917 0.15623333 0.12153333 -0.03470000 0.749807473 0.003817998 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000135476 E001 0.3579807 2.822690e-02 3.040212e-01   12 53268261 53268262 2 + 0.186 0.000 -9.499
ENSG00000135476 E002 15.0235758 5.460074e-03 2.746339e-02 0.2689388567 12 53268263 53268298 36 + 1.277 1.076 -0.714
ENSG00000135476 E003 63.9362408 4.689750e-04 4.580472e-05 0.0083777641 12 53268299 53268332 34 + 1.878 1.698 -0.606
ENSG00000135476 E004 70.2031111 2.316625e-03 1.013597e-03 0.0545363728 12 53268333 53268334 2 + 1.908 1.757 -0.509
ENSG00000135476 E005 105.7768806 3.081499e-03 2.634216e-03 0.0920683363 12 53268335 53268377 43 + 2.075 1.952 -0.411
ENSG00000135476 E006 26.1494207 3.416869e-02 7.972962e-01 0.9474273385 12 53268378 53268406 29 + 1.439 1.417 -0.078
ENSG00000135476 E007 173.0082938 3.699230e-03 5.319132e-01 0.8460148975 12 53268755 53268847 93 + 2.245 2.228 -0.058
ENSG00000135476 E008 15.1098007 2.006178e-02 9.137318e-01 0.9793793461 12 53268848 53268928 81 + 1.190 1.216 0.093
ENSG00000135476 E009 422.0163245 3.361194e-03 6.545543e-02 0.3855483056 12 53269024 53269525 502 + 2.645 2.593 -0.171
ENSG00000135476 E010 240.0493643 9.859961e-04 9.177373e-02 0.4416728284 12 53269526 53269701 176 + 2.398 2.356 -0.139
ENSG00000135476 E011 427.7589534 7.862467e-04 5.626592e-01 0.8590508972 12 53269702 53270085 384 + 2.632 2.625 -0.022
ENSG00000135476 E012 230.6961044 5.330045e-03 2.732319e-01 0.6770714422 12 53270378 53270482 105 + 2.379 2.339 -0.134
ENSG00000135476 E013 259.5436193 2.361415e-03 1.961729e-01 0.5992959276 12 53270678 53270798 121 + 2.425 2.394 -0.104
ENSG00000135476 E014 298.2076397 3.276314e-04 3.823253e-01 0.7617041868 12 53272721 53272857 137 + 2.478 2.465 -0.042
ENSG00000135476 E015 39.2944665 1.218363e-01 3.751468e-02 0.3056444645 12 53274081 53274466 386 + 1.442 1.741 1.020
ENSG00000135476 E016 321.6569739 1.506348e-04 4.955330e-01 0.8271632033 12 53274817 53275010 194 + 2.509 2.501 -0.027
ENSG00000135476 E017 366.4347747 1.010086e-04 1.485246e-02 0.2075724972 12 53276620 53276859 240 + 2.578 2.538 -0.132
ENSG00000135476 E018 277.6066224 1.572683e-04 1.816221e-01 0.5814496903 12 53277083 53277227 145 + 2.450 2.427 -0.077
ENSG00000135476 E019 289.2514821 1.005939e-03 6.169891e-01 0.8828000925 12 53277470 53277608 139 + 2.461 2.454 -0.023
ENSG00000135476 E020 254.0788241 2.590350e-04 4.286238e-01 0.7911724395 12 53277821 53277960 140 + 2.410 2.397 -0.042
ENSG00000135476 E021 1.0888155 1.335999e-02 9.550981e-01   12 53279705 53279731 27 + 0.315 0.328 0.081
ENSG00000135476 E022 278.6903517 8.828997e-04 5.725106e-01 0.8632783999 12 53279732 53279866 135 + 2.435 2.455 0.066
ENSG00000135476 E023 296.2357037 1.499218e-04 8.375327e-01 0.9595302074 12 53281507 53281626 120 + 2.468 2.470 0.006
ENSG00000135476 E024 0.6936514 1.183113e-01 5.066161e-01   12 53282250 53282263 14 + 0.260 0.138 -1.128
ENSG00000135476 E025 349.9860157 1.271741e-04 6.685529e-01 0.9036429772 12 53282264 53282435 172 + 2.533 2.547 0.049
ENSG00000135476 E026 336.0527604 1.207629e-04 1.276998e-01 0.5067678248 12 53283129 53283257 129 + 2.506 2.542 0.120
ENSG00000135476 E027 329.0225015 1.373526e-04 7.955078e-01 0.9466456408 12 53283382 53283538 157 + 2.515 2.516 0.003
ENSG00000135476 E028 252.4838959 1.539195e-03 4.754425e-01 0.8168996246 12 53284058 53284167 110 + 2.405 2.393 -0.040
ENSG00000135476 E029 1057.5162352 1.017171e-04 3.204535e-02 0.2875002457 12 53285924 53286912 989 + 3.028 3.010 -0.059
ENSG00000135476 E030 46.5906400 4.508892e-02 3.813723e-04 0.0304711853 12 53287256 53287880 625 + 1.443 1.851 1.388
ENSG00000135476 E031 24.6703939 1.690960e-02 1.463962e-06 0.0007580992 12 53287881 53287971 91 + 1.138 1.596 1.595
ENSG00000135476 E032 735.0251082 5.603843e-04 3.646065e-01 0.7500598603 12 53287972 53288341 370 + 2.866 2.860 -0.022
ENSG00000135476 E033 445.6820340 8.712609e-04 6.939679e-01 0.9132379288 12 53288538 53288699 162 + 2.647 2.647 -0.001
ENSG00000135476 E034 30.7477339 1.525941e-01 3.287123e-02 0.2903754926 12 53288700 53289089 390 + 1.300 1.658 1.234
ENSG00000135476 E035 557.1249831 3.634205e-04 3.825439e-01 0.7618964811 12 53289090 53289303 214 + 2.733 2.755 0.073
ENSG00000135476 E036 538.4479309 1.632623e-04 8.865648e-01 0.9728029347 12 53289404 53289594 191 + 2.724 2.733 0.030
ENSG00000135476 E037 179.0667909 7.181966e-02 4.561151e-02 0.3317900594 12 53289595 53290084 490 + 2.140 2.361 0.739
ENSG00000135476 E038 459.4093812 1.838892e-03 6.910059e-01 0.9121798631 12 53290085 53290212 128 + 2.658 2.657 -0.003
ENSG00000135476 E039 83.2220978 5.052433e-02 6.820150e-03 0.1455748682 12 53290213 53290346 134 + 1.766 2.059 0.984
ENSG00000135476 E040 455.8589218 4.002271e-03 3.778225e-01 0.7585222406 12 53290347 53290469 123 + 2.637 2.680 0.142
ENSG00000135476 E041 450.3723263 2.896111e-03 8.712505e-01 0.9684896770 12 53290841 53290996 156 + 2.650 2.654 0.012
ENSG00000135476 E042 561.0520230 9.097111e-05 9.829978e-01 0.9958402596 12 53291690 53291860 171 + 2.743 2.749 0.021
ENSG00000135476 E043 474.6042852 1.070048e-03 9.488850e-01 0.9880335874 12 53291984 53292088 105 + 2.669 2.676 0.021
ENSG00000135476 E044 523.5116597 1.075874e-03 3.522198e-01 0.7412201730 12 53292278 53292393 116 + 2.701 2.730 0.097
ENSG00000135476 E045 382.9150702 3.848372e-03 3.387054e-01 0.7310379917 12 53292574 53292657 84 + 2.558 2.606 0.161
ENSG00000135476 E046 396.0445667 6.618524e-04 3.050538e-01 0.7039430991 12 53292806 53292970 165 + 2.580 2.610 0.101
ENSG00000135476 E047 481.2604427 3.517522e-04 1.265191e-01 0.5049825584 12 53293273 53293638 366 + 2.664 2.698 0.113