ENSG00000134186

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370025 ENSG00000134186 HEK293_DMSO_6hA HEK293_OSMI2_6hA PRPF38B protein_coding protein_coding 46.44651 21.12494 19.12522 4.840376 1.520603 -0.1433998 20.337566 8.0744041 8.9940645 1.66840821 1.0945993 0.1554350 0.47777083 0.38570000 0.47623333 0.09053333 0.62307419 0.01086016 FALSE TRUE
ENST00000467302 ENSG00000134186 HEK293_DMSO_6hA HEK293_OSMI2_6hA PRPF38B protein_coding processed_transcript 46.44651 21.12494 19.12522 4.840376 1.520603 -0.1433998 5.764553 3.0794141 2.0972031 0.75927765 0.5141624 -0.5520038 0.11643333 0.14473333 0.10820000 -0.03653333 0.65139953 0.01086016 TRUE FALSE
ENST00000485810 ENSG00000134186 HEK293_DMSO_6hA HEK293_OSMI2_6hA PRPF38B protein_coding processed_transcript 46.44651 21.12494 19.12522 4.840376 1.520603 -0.1433998 8.560532 6.0184050 2.8023363 1.64364257 0.6710864 -1.1000072 0.17708750 0.28113333 0.14506667 -0.13606667 0.08048734 0.01086016 FALSE FALSE
MSTRG.1723.14 ENSG00000134186 HEK293_DMSO_6hA HEK293_OSMI2_6hA PRPF38B protein_coding   46.44651 21.12494 19.12522 4.840376 1.520603 -0.1433998 5.094298 0.1947043 0.7184607 0.19470433 0.3737781 1.8313096 0.07482917 0.01153333 0.03486667 0.02333333 0.65625636 0.01086016 TRUE FALSE
MSTRG.1723.16 ENSG00000134186 HEK293_DMSO_6hA HEK293_OSMI2_6hA PRPF38B protein_coding   46.44651 21.12494 19.12522 4.840376 1.520603 -0.1433998 1.567844 0.6760143 2.2126365 0.27747943 0.1685071 1.6959614 0.04567083 0.03623333 0.11586667 0.07963333 0.11972284 0.01086016 FALSE FALSE
MSTRG.1723.4 ENSG00000134186 HEK293_DMSO_6hA HEK293_OSMI2_6hA PRPF38B protein_coding   46.44651 21.12494 19.12522 4.840376 1.520603 -0.1433998 2.637592 0.1583557 1.4313672 0.08614971 0.1867271 3.0978536 0.04789583 0.00780000 0.07450000 0.06670000 0.01086016 0.01086016 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000134186 E001 0.000000       1 108691836 108692252 417 +      
ENSG00000134186 E002 0.000000       1 108692253 108692272 20 +      
ENSG00000134186 E003 0.000000       1 108692273 108692304 32 +      
ENSG00000134186 E004 1.787530 0.0221610710 2.393105e-01 0.644540553 1 108692305 108692309 5 + 0.527 0.341 -0.990
ENSG00000134186 E005 5.380242 0.0047410497 1.731405e-01 0.570691641 1 108692310 108692323 14 + 0.673 0.878 0.822
ENSG00000134186 E006 5.917796 0.0045269022 2.779110e-01 0.681227785 1 108692324 108692326 3 + 0.731 0.895 0.644
ENSG00000134186 E007 13.766089 0.0020136894 5.134337e-01 0.836887330 1 108692327 108692334 8 + 1.190 1.159 -0.111
ENSG00000134186 E008 19.898518 0.0013534393 4.317622e-01 0.792877368 1 108692335 108692340 6 + 1.340 1.309 -0.109
ENSG00000134186 E009 31.802166 0.0009094836 3.871537e-02 0.309735815 1 108692341 108692363 23 + 1.569 1.471 -0.333
ENSG00000134186 E010 33.085749 0.0008902466 7.039161e-02 0.396509720 1 108692364 108692369 6 + 1.577 1.497 -0.273
ENSG00000134186 E011 51.991271 0.0028698663 1.039130e-02 0.176572136 1 108692370 108692450 81 + 1.784 1.671 -0.384
ENSG00000134186 E012 57.089619 0.0014061334 1.051824e-04 0.013910350 1 108692451 108692524 74 + 1.845 1.685 -0.543
ENSG00000134186 E013 154.530673 0.0014415422 4.213643e-06 0.001650682 1 108692525 108692867 343 + 2.254 2.129 -0.416
ENSG00000134186 E014 6.831080 0.2020944516 9.208256e-01 0.981040621 1 108693590 108693642 53 + 0.891 0.887 -0.015
ENSG00000134186 E015 93.905529 0.0010941910 8.262915e-03 0.158727405 1 108695702 108695770 69 + 2.010 1.937 -0.247
ENSG00000134186 E016 9.391995 0.0122210606 2.979271e-01 0.698172893 1 108695771 108695780 10 + 0.926 1.069 0.528
ENSG00000134186 E017 112.021015 0.0002740571 4.418870e-04 0.033481782 1 108696043 108696194 152 + 2.095 2.009 -0.289
ENSG00000134186 E018 66.370348 0.0007424085 1.628269e-01 0.557605335 1 108696277 108696337 61 + 1.844 1.811 -0.108
ENSG00000134186 E019 12.530074 0.0601361158 1.429597e-01 0.529402743 1 108696338 108696351 14 + 1.000 1.201 0.727
ENSG00000134186 E020 46.853896 0.0337204315 3.130601e-02 0.284646805 1 108696352 108696739 388 + 1.537 1.757 0.747
ENSG00000134186 E021 107.076919 0.0178983572 1.279253e-02 0.194934590 1 108696740 108697504 765 + 1.914 2.102 0.629
ENSG00000134186 E022 24.069597 0.0093999886 3.917997e-03 0.111919900 1 108697700 108697700 1 + 1.221 1.492 0.943
ENSG00000134186 E023 47.787048 0.0179317540 4.340246e-03 0.118215702 1 108697701 108697809 109 + 1.527 1.776 0.844
ENSG00000134186 E024 39.003480 0.0199949823 4.108630e-02 0.317632421 1 108697810 108697863 54 + 1.473 1.677 0.697
ENSG00000134186 E025 43.511428 0.0162210419 5.499366e-04 0.038217386 1 108697864 108697946 83 + 1.440 1.754 1.072
ENSG00000134186 E026 53.076935 0.0234163611 1.702654e-02 0.220805364 1 108697947 108698017 71 + 1.589 1.813 0.760
ENSG00000134186 E027 81.406576 0.0325262271 2.249867e-03 0.084463788 1 108698018 108698603 586 + 1.724 2.017 0.986
ENSG00000134186 E028 222.671633 0.0001606511 1.243288e-02 0.192419924 1 108698604 108698827 224 + 2.362 2.334 -0.094
ENSG00000134186 E029 327.727146 0.0001442017 1.395693e-01 0.524531990 1 108699162 108699466 305 + 2.515 2.517 0.006
ENSG00000134186 E030 231.426982 0.0001944200 9.154757e-01 0.979813480 1 108699467 108699570 104 + 2.348 2.380 0.106
ENSG00000134186 E031 959.133160 0.0106614598 9.608134e-01 0.991027609 1 108699571 108702928 3358 + 2.963 2.992 0.096