ENSG00000130734

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000309469 ENSG00000130734 HEK293_DMSO_6hA HEK293_OSMI2_6hA ATG4D protein_coding protein_coding 57.22854 46.88494 86.05485 4.772455 19.04279 0.875992 22.109119 14.847118 35.967049 2.1296598 8.5797715 1.2759225 0.39504167 0.31380000 0.41430000 0.10050000 0.006954945 0.006954945 FALSE TRUE
ENST00000586417 ENSG00000130734 HEK293_DMSO_6hA HEK293_OSMI2_6hA ATG4D protein_coding nonsense_mediated_decay 57.22854 46.88494 86.05485 4.772455 19.04279 0.875992 5.767703 3.679760 10.480293 0.4679297 2.7793958 1.5074559 0.09700000 0.07823333 0.11836667 0.04013333 0.125482276 0.006954945 FALSE TRUE
ENST00000586863 ENSG00000130734 HEK293_DMSO_6hA HEK293_OSMI2_6hA ATG4D protein_coding protein_coding 57.22854 46.88494 86.05485 4.772455 19.04279 0.875992 2.707271 4.198604 3.767781 0.9981829 0.7490986 -0.1558027 0.05255417 0.08806667 0.04956667 -0.03850000 0.404824565 0.006954945 FALSE FALSE
ENST00000588972 ENSG00000130734 HEK293_DMSO_6hA HEK293_OSMI2_6hA ATG4D protein_coding protein_coding 57.22854 46.88494 86.05485 4.772455 19.04279 0.875992 6.758621 10.186336 6.959071 0.8197542 0.6506332 -0.5490126 0.13174583 0.22243333 0.08920000 -0.13323333 0.008602322 0.006954945 FALSE TRUE
ENST00000589753 ENSG00000130734 HEK293_DMSO_6hA HEK293_OSMI2_6hA ATG4D protein_coding nonsense_mediated_decay 57.22854 46.88494 86.05485 4.772455 19.04279 0.875992 8.482975 5.195267 11.242401 1.6833762 3.1060995 1.1121889 0.12000000 0.10933333 0.12753333 0.01820000 0.753172310 0.006954945 FALSE TRUE
MSTRG.16415.7 ENSG00000130734 HEK293_DMSO_6hA HEK293_OSMI2_6hA ATG4D protein_coding   57.22854 46.88494 86.05485 4.772455 19.04279 0.875992 3.841630 2.403345 8.669090 1.1151529 2.8039234 1.8465098 0.05712500 0.05260000 0.09503333 0.04243333 0.542954687 0.006954945 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000130734 E001 3.002505 0.5115616501 0.3702381166 0.75366907 19 10543895 10543903 9 + 0.677 0.368 -1.496
ENSG00000130734 E002 15.292918 0.0018342268 0.0262049784 0.26320771 19 10543904 10543914 11 + 1.268 1.063 -0.732
ENSG00000130734 E003 17.338315 0.0016707755 0.0129594559 0.19595752 19 10543915 10543916 2 + 1.328 1.110 -0.770
ENSG00000130734 E004 17.696296 0.0015759016 0.0090915438 0.16613041 19 10543917 10543917 1 + 1.337 1.110 -0.802
ENSG00000130734 E005 23.013917 0.0012203243 0.0238781520 0.25387216 19 10543918 10543921 4 + 1.431 1.259 -0.597
ENSG00000130734 E006 54.310236 0.0009854216 0.0004830282 0.03532309 19 10543922 10543970 49 + 1.794 1.614 -0.611
ENSG00000130734 E007 263.040842 0.0001370406 0.0011877045 0.05971595 19 10543971 10544282 312 + 2.437 2.366 -0.238
ENSG00000130734 E008 171.597976 0.0002104361 0.3258280898 0.72149539 19 10544283 10544308 26 + 2.235 2.210 -0.085
ENSG00000130734 E009 178.631029 0.0002307326 0.6093824874 0.87971535 19 10544309 10544320 12 + 2.247 2.235 -0.039
ENSG00000130734 E010 172.055054 0.0002152846 0.5053673286 0.83236894 19 10544321 10544325 5 + 2.232 2.216 -0.054
ENSG00000130734 E011 59.508554 0.0108349718 0.8403555635 0.96040884 19 10544709 10544782 74 + 1.762 1.778 0.055
ENSG00000130734 E012 361.966407 0.0006171111 0.4496864367 0.80343498 19 10544783 10544866 84 + 2.537 2.561 0.079
ENSG00000130734 E013 3.919229 0.0059744149 0.0193568722 0.23251784 19 10544867 10544956 90 + 0.516 0.855 1.433
ENSG00000130734 E014 304.968881 0.0001650068 0.6433820808 0.89369469 19 10544957 10544996 40 + 2.469 2.482 0.042
ENSG00000130734 E015 247.622529 0.0001717528 0.6629480573 0.90146030 19 10544997 10545009 13 + 2.379 2.391 0.042
ENSG00000130734 E016 444.083030 0.0001118574 0.5448749655 0.85201348 19 10545010 10545119 110 + 2.640 2.632 -0.025
ENSG00000130734 E017 217.221086 0.0009337828 0.3957041140 0.77040646 19 10545120 10545130 11 + 2.330 2.315 -0.050
ENSG00000130734 E018 453.176711 0.0002243766 0.2902117014 0.69151236 19 10546839 10547071 233 + 2.654 2.638 -0.052
ENSG00000130734 E019 167.069649 0.0002713199 0.6904728268 0.91194363 19 10547072 10547082 11 + 2.222 2.212 -0.032
ENSG00000130734 E020 188.039097 0.0004565531 0.8884421698 0.97336705 19 10547083 10547115 33 + 2.269 2.268 -0.003
ENSG00000130734 E021 30.737943 0.1177342594 0.2501601903 0.65593631 19 10547116 10547188 73 + 1.395 1.608 0.732
ENSG00000130734 E022 248.209660 0.0029319319 0.7021727262 0.91585993 19 10547189 10547253 65 + 2.385 2.384 -0.001
ENSG00000130734 E023 410.694575 0.0001109875 0.2935762527 0.69431012 19 10548904 10549028 125 + 2.611 2.594 -0.056
ENSG00000130734 E024 221.185313 0.0001612956 0.8011962957 0.94870077 19 10549029 10549034 6 + 2.336 2.332 -0.013
ENSG00000130734 E025 286.448177 0.0001493009 0.1450026425 0.53220972 19 10551897 10551935 39 + 2.457 2.429 -0.096
ENSG00000130734 E026 279.226180 0.0001605242 0.0522493229 0.35068000 19 10551936 10551975 40 + 2.450 2.410 -0.134
ENSG00000130734 E027 23.832718 0.0371393610 0.0062161910 0.13964274 19 10551976 10552044 69 + 1.223 1.556 1.153
ENSG00000130734 E028 424.438813 0.0001315063 0.2398298598 0.64501456 19 10552045 10552121 77 + 2.610 2.633 0.077
ENSG00000130734 E029 579.341880 0.0001013702 0.0044514720 0.11961229 19 10552205 10552324 120 + 2.736 2.781 0.150
ENSG00000130734 E030 991.891436 0.0006791792 0.0012898989 0.06265336 19 10552885 10553418 534 + 2.963 3.019 0.187