ENSG00000127419

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264771 ENSG00000127419 HEK293_DMSO_6hA HEK293_OSMI2_6hA TMEM175 protein_coding protein_coding 28.2346 38.10298 41.97219 1.59473 1.495636 0.1394948 5.710486 4.730986 10.298361 0.3827703 0.7371039 1.1205559 0.18971667 0.12383333 0.24690000 0.123066667 0.0005981661 0.0005981661 FALSE TRUE
ENST00000452360 ENSG00000127419 HEK293_DMSO_6hA HEK293_OSMI2_6hA TMEM175 protein_coding retained_intron 28.2346 38.10298 41.97219 1.59473 1.495636 0.1394948 1.661809 3.365791 2.329695 0.4417538 0.3490065 -0.5289049 0.05922917 0.08893333 0.05516667 -0.033766667 0.3774004652 0.0005981661 FALSE TRUE
ENST00000506669 ENSG00000127419 HEK293_DMSO_6hA HEK293_OSMI2_6hA TMEM175 protein_coding retained_intron 28.2346 38.10298 41.97219 1.59473 1.495636 0.1394948 1.981489 2.507425 4.023623 1.5462436 1.6412719 0.6801273 0.06423333 0.06286667 0.09423333 0.031366667 0.8363319351 0.0005981661   FALSE
ENST00000508204 ENSG00000127419 HEK293_DMSO_6hA HEK293_OSMI2_6hA TMEM175 protein_coding protein_coding 28.2346 38.10298 41.97219 1.59473 1.495636 0.1394948 2.141197 2.888158 2.664299 0.4427668 1.3372431 -0.1159755 0.07480417 0.07520000 0.06583333 -0.009366667 0.8570179861 0.0005981661 FALSE TRUE
ENST00000515876 ENSG00000127419 HEK293_DMSO_6hA HEK293_OSMI2_6hA TMEM175 protein_coding retained_intron 28.2346 38.10298 41.97219 1.59473 1.495636 0.1394948 3.417648 4.935748 3.176021 0.6484538 1.0495408 -0.6344330 0.13540417 0.12916667 0.07416667 -0.055000000 0.3291838098 0.0005981661 FALSE TRUE
MSTRG.24429.1 ENSG00000127419 HEK293_DMSO_6hA HEK293_OSMI2_6hA TMEM175 protein_coding   28.2346 38.10298 41.97219 1.59473 1.495636 0.1394948 2.711492 1.141115 4.919108 0.5818271 1.2265454 2.0982948 0.09654167 0.03126667 0.11950000 0.088233333 0.4538944714 0.0005981661 FALSE TRUE
MSTRG.24429.16 ENSG00000127419 HEK293_DMSO_6hA HEK293_OSMI2_6hA TMEM175 protein_coding   28.2346 38.10298 41.97219 1.59473 1.495636 0.1394948 2.920087 5.085105 3.564876 1.1750244 0.8692641 -0.5112187 0.10079583 0.13260000 0.08370000 -0.048900000 0.5999922348 0.0005981661 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000127419 E001 1.4434310 0.0174734225 4.264203e-01   4 932387 932403 17 + 0.440 0.308 -0.768
ENSG00000127419 E002 3.2406449 0.0064767997 9.797313e-02 0.45352918 4 932404 932425 22 + 0.731 0.486 -1.086
ENSG00000127419 E003 4.5576170 0.0047993636 9.456867e-02 0.44731220 4 932426 932431 6 + 0.835 0.612 -0.918
ENSG00000127419 E004 6.1169297 0.0037402368 4.878109e-02 0.34093065 4 932432 932443 12 + 0.949 0.710 -0.935
ENSG00000127419 E005 6.6983063 0.0033630575 3.240961e-02 0.28876029 4 932444 932445 2 + 0.990 0.738 -0.971
ENSG00000127419 E006 9.8779113 0.0023706257 3.701434e-02 0.30390870 4 932446 932459 14 + 1.123 0.915 -0.765
ENSG00000127419 E007 10.3260986 0.0022947464 3.284168e-02 0.29035701 4 932460 932460 1 + 1.142 0.933 -0.765
ENSG00000127419 E008 10.3260986 0.0022947464 3.284168e-02 0.29035701 4 932461 932461 1 + 1.142 0.933 -0.765
ENSG00000127419 E009 12.1943327 0.0020495036 3.678375e-02 0.30310289 4 932462 932464 3 + 1.201 1.012 -0.682
ENSG00000127419 E010 21.0536473 0.0013396203 4.107085e-02 0.31759039 4 932465 932471 7 + 1.402 1.260 -0.496
ENSG00000127419 E011 24.8811799 0.0011175930 3.874085e-02 0.30975668 4 932472 932473 2 + 1.468 1.335 -0.458
ENSG00000127419 E012 31.0728369 0.0009679812 7.399925e-02 0.40415127 4 932474 932474 1 + 1.550 1.450 -0.343
ENSG00000127419 E013 45.3511997 0.0006592751 2.984072e-02 0.27889885 4 932475 932477 3 + 1.710 1.608 -0.346
ENSG00000127419 E014 52.9485385 0.0008094953 7.683539e-02 0.41098500 4 932478 932479 2 + 1.763 1.688 -0.254
ENSG00000127419 E015 58.1369205 0.0005615830 1.227948e-02 0.19131451 4 932480 932481 2 + 1.817 1.712 -0.353
ENSG00000127419 E016 66.3825690 0.0004714676 1.979623e-02 0.23457771 4 932482 932486 5 + 1.866 1.777 -0.301
ENSG00000127419 E017 108.5938279 0.0002953782 6.912806e-02 0.39375362 4 932487 932516 30 + 2.061 2.011 -0.168
ENSG00000127419 E018 118.7567671 0.0002505403 9.543362e-02 0.44903649 4 932517 932540 24 + 2.095 2.052 -0.142
ENSG00000127419 E019 0.1767706 0.0332145262 5.468130e-01   4 945999 946126 128 + 0.112 0.000 -9.080
ENSG00000127419 E020 174.8127299 0.0002061924 6.575152e-03 0.14294478 4 947709 947892 184 + 2.270 2.209 -0.203
ENSG00000127419 E021 53.0886318 0.0687248822 8.573718e-02 0.42950097 4 947893 948115 223 + 1.632 1.814 0.615
ENSG00000127419 E022 121.7987777 0.0002591939 5.963081e-01 0.87409288 4 948116 948154 39 + 2.087 2.082 -0.019
ENSG00000127419 E023 51.2886283 0.0957822825 1.088910e-01 0.47352067 4 948155 948410 256 + 1.615 1.803 0.637
ENSG00000127419 E024 31.9709511 0.1333466766 2.075136e-01 0.61143082 4 948411 948510 100 + 1.410 1.607 0.678
ENSG00000127419 E025 32.9358298 0.0689834379 3.005821e-01 0.70042166 4 948511 948615 105 + 1.458 1.592 0.457
ENSG00000127419 E026 105.2445595 0.0003378150 7.399960e-01 0.92913288 4 950421 950423 3 + 2.019 2.019 0.000
ENSG00000127419 E027 137.6375430 0.0002258902 5.147781e-01 0.83748950 4 950424 950450 27 + 2.141 2.133 -0.027
ENSG00000127419 E028 184.4056884 0.0001994811 5.504534e-01 0.85413721 4 950451 950518 68 + 2.267 2.263 -0.014
ENSG00000127419 E029 186.7404556 0.0004560582 2.041600e-01 0.60810541 4 951207 951258 52 + 2.246 2.291 0.151
ENSG00000127419 E030 1.1486300 0.0166459960 8.482861e-01   4 951259 951259 1 + 0.337 0.308 -0.182
ENSG00000127419 E031 29.8477629 0.0023445427 1.303840e-02 0.19646998 4 951646 951681 36 + 1.397 1.569 0.593
ENSG00000127419 E032 171.2755337 0.0004945776 1.337467e-01 0.51583988 4 951682 951717 36 + 2.206 2.259 0.177
ENSG00000127419 E033 12.6761410 0.0037056231 1.579022e-04 0.01767350 4 951718 951973 256 + 0.904 1.284 1.377
ENSG00000127419 E034 127.4239481 0.0002906279 1.040808e-01 0.46495727 4 952367 952410 44 + 2.074 2.135 0.206
ENSG00000127419 E035 109.6712846 0.0010487311 7.990136e-01 0.94801568 4 952411 952450 40 + 2.039 2.041 0.004
ENSG00000127419 E036 294.5495929 0.0006403153 5.575986e-01 0.85717975 4 953190 953354 165 + 2.453 2.478 0.083
ENSG00000127419 E037 219.8180746 0.0010359977 6.038996e-01 0.87725922 4 955405 955483 79 + 2.324 2.351 0.089
ENSG00000127419 E038 6.0287822 0.0188618682 5.274935e-01 0.84368918 4 955484 955491 8 + 0.779 0.878 0.385
ENSG00000127419 E039 282.7074391 0.0056910713 7.285310e-01 0.92526415 4 955755 955890 136 + 2.450 2.452 0.007
ENSG00000127419 E040 19.5991149 0.0372613680 2.227887e-02 0.24694784 4 955891 955944 54 + 1.146 1.425 0.980
ENSG00000127419 E041 83.9741238 0.0095700998 7.101417e-06 0.00240648 4 956297 956892 596 + 1.770 2.047 0.931
ENSG00000127419 E042 592.7528588 0.0038800518 5.437993e-01 0.85162878 4 957824 958656 833 + 2.773 2.772 -0.006