ENSG00000125458

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000245552 ENSG00000125458 HEK293_DMSO_6hA HEK293_OSMI2_6hA NT5C protein_coding protein_coding 94.27696 77.3749 133.769 6.781898 29.7304 0.7897278 54.075265 28.317500 79.614110 3.86397839 23.5988325 1.491002056 0.52841250 0.36663333 0.56840000 0.20176667 0.12264945 0.03829724 FALSE  
ENST00000578337 ENSG00000125458 HEK293_DMSO_6hA HEK293_OSMI2_6hA NT5C protein_coding protein_coding 94.27696 77.3749 133.769 6.781898 29.7304 0.7897278 4.496820 5.853092 6.617650 0.09649689 0.7845369 0.176835546 0.05416667 0.07696667 0.05246667 -0.02450000 0.33433690 0.03829724    
ENST00000580423 ENSG00000125458 HEK293_DMSO_6hA HEK293_OSMI2_6hA NT5C protein_coding protein_coding 94.27696 77.3749 133.769 6.781898 29.7304 0.7897278 5.132935 2.858704 8.878105 0.38033045 1.5610388 1.631476599 0.05047917 0.03783333 0.06826667 0.03043333 0.17007433 0.03829724    
ENST00000582160 ENSG00000125458 HEK293_DMSO_6hA HEK293_OSMI2_6hA NT5C protein_coding protein_coding 94.27696 77.3749 133.769 6.781898 29.7304 0.7897278 6.712488 9.389275 7.140096 1.94402561 2.9106589 -0.394586703 0.07693750 0.11946667 0.05130000 -0.06816667 0.03829724 0.03829724 FALSE  
ENST00000583655 ENSG00000125458 HEK293_DMSO_6hA HEK293_OSMI2_6hA NT5C protein_coding retained_intron 94.27696 77.3749 133.769 6.781898 29.7304 0.7897278 4.192184 5.910017 5.929208 0.32050121 0.5418151 0.004669162 0.05380833 0.07683333 0.05083333 -0.02600000 0.43706081 0.03829724    
ENST00000584352 ENSG00000125458 HEK293_DMSO_6hA HEK293_OSMI2_6hA NT5C protein_coding retained_intron 94.27696 77.3749 133.769 6.781898 29.7304 0.7897278 10.026206 15.365599 12.609022 1.71577318 3.0209072 -0.285042538 0.13764583 0.19896667 0.10860000 -0.09036667 0.28755047 0.03829724    
MSTRG.15053.9 ENSG00000125458 HEK293_DMSO_6hA HEK293_OSMI2_6hA NT5C protein_coding   94.27696 77.3749 133.769 6.781898 29.7304 0.7897278 5.482637 5.471164 6.738325 1.93718116 1.4386754 0.300047018 0.05574167 0.06943333 0.05113333 -0.01830000 0.78995774 0.03829724    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000125458 E001 2.340693 5.905361e-02 0.7696530379 0.93940302 17 75130225 75130227 3 - 0.517 0.479 -0.183
ENSG00000125458 E002 7.130715 3.640474e-03 0.2748575422 0.67853717 17 75130228 75130229 2 - 0.845 0.975 0.494
ENSG00000125458 E003 142.003399 2.422641e-04 0.0744078078 0.40520699 17 75130230 75130270 41 - 2.134 2.188 0.180
ENSG00000125458 E004 222.123341 1.885783e-04 0.8410959455 0.96069181 17 75130271 75130331 61 - 2.351 2.347 -0.012
ENSG00000125458 E005 258.982007 2.360080e-04 0.3578242218 0.74532488 17 75130332 75130362 31 - 2.425 2.405 -0.066
ENSG00000125458 E006 453.460963 3.505702e-04 0.8348064125 0.95851593 17 75130363 75130435 73 - 2.655 2.662 0.024
ENSG00000125458 E007 470.696478 8.776892e-05 0.0248075413 0.25775151 17 75130436 75130472 37 - 2.688 2.652 -0.117
ENSG00000125458 E008 738.511612 1.560385e-04 0.3868637599 0.76501002 17 75130473 75130545 73 - 2.870 2.863 -0.026
ENSG00000125458 E009 575.665531 4.416377e-04 0.9417406023 0.98640331 17 75130546 75130567 22 - 2.756 2.764 0.025
ENSG00000125458 E010 460.917730 1.825701e-04 0.7633570751 0.93705655 17 75130568 75130570 3 - 2.662 2.669 0.024
ENSG00000125458 E011 734.659143 7.798533e-05 0.5519815040 0.85493983 17 75130571 75130637 67 - 2.863 2.872 0.032
ENSG00000125458 E012 429.464374 1.103305e-04 0.0166661816 0.21856096 17 75130638 75130642 5 - 2.615 2.658 0.142
ENSG00000125458 E013 97.028185 1.298134e-02 0.0007415750 0.04583575 17 75130643 75130752 110 - 1.864 2.113 0.835
ENSG00000125458 E014 566.693421 6.423925e-04 0.1841130005 0.58448183 17 75130753 75130787 35 - 2.735 2.770 0.114
ENSG00000125458 E015 680.539529 4.527131e-04 0.9623269237 0.99135622 17 75130788 75130867 80 - 2.828 2.834 0.020
ENSG00000125458 E016 107.973016 6.538453e-02 0.0307777805 0.28235372 17 75130868 75130964 97 - 1.885 2.179 0.987
ENSG00000125458 E017 132.716263 3.005963e-02 0.0381932600 0.30759338 17 75130965 75131044 80 - 2.023 2.228 0.686
ENSG00000125458 E018 429.866606 1.935507e-03 0.0387356405 0.30974877 17 75131045 75131081 37 - 2.650 2.602 -0.158
ENSG00000125458 E019 349.379573 1.086342e-03 0.0006724241 0.04347512 17 75131082 75131105 24 - 2.574 2.495 -0.262
ENSG00000125458 E020 68.109295 6.125125e-02 0.1120908927 0.47944413 17 75131106 75131180 75 - 1.723 1.951 0.769
ENSG00000125458 E021 525.643784 1.613217e-03 0.0011190399 0.05773194 17 75131181 75131281 101 - 2.746 2.675 -0.237
ENSG00000125458 E022 155.342109 3.213270e-02 0.0162363316 0.21606669 17 75131282 75131533 252 - 2.072 2.312 0.802
ENSG00000125458 E023 284.904160 1.061676e-02 0.0006058561 0.04071201 17 75131534 75131757 224 - 2.513 2.343 -0.567