ENSG00000122034

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000381140 ENSG00000122034 HEK293_DMSO_6hA HEK293_OSMI2_6hA GTF3A protein_coding protein_coding 87.77941 52.28563 53.2552 9.570238 2.429006 0.02650276 76.967709 41.329707 47.7816981 7.8609474 2.8216829 0.2092319 0.87380000 0.7886333 0.896166667 0.10753333 1.435156e-05 1.435156e-05 FALSE TRUE
ENST00000482655 ENSG00000122034 HEK293_DMSO_6hA HEK293_OSMI2_6hA GTF3A protein_coding retained_intron 87.77941 52.28563 53.2552 9.570238 2.429006 0.02650276 1.474623 3.457114 0.5211864 0.6597339 0.2110095 -2.7064452 0.02064167 0.0662000 0.009466667 -0.05673333 1.706854e-04 1.435156e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000122034 E001 1.751671 2.465070e-01 2.790703e-01 0.682454152 13 27424616 27424618 3 + 0.591 0.293 -1.589
ENSG00000122034 E002 21.603455 1.180944e-02 8.813481e-05 0.012611630 13 27424619 27424637 19 + 1.509 1.152 -1.248
ENSG00000122034 E003 33.151446 2.096724e-02 2.015219e-04 0.020550149 13 27424638 27424661 24 + 1.685 1.334 -1.204
ENSG00000122034 E004 42.739544 1.622885e-02 9.554481e-04 0.052565191 13 27424662 27424675 14 + 1.765 1.490 -0.934
ENSG00000122034 E005 117.688108 5.999426e-03 2.212749e-04 0.021664088 13 27424676 27424765 90 + 2.161 1.977 -0.619
ENSG00000122034 E006 211.000136 3.645805e-03 2.630162e-02 0.263579641 13 27424766 27424853 88 + 2.370 2.282 -0.294
ENSG00000122034 E007 264.218444 3.100600e-03 6.053040e-02 0.372815201 13 27424854 27424938 85 + 2.457 2.389 -0.228
ENSG00000122034 E008 11.568124 9.973377e-02 2.891562e-01 0.690750626 13 27424939 27425165 227 + 1.020 1.151 0.477
ENSG00000122034 E009 39.344980 9.810946e-02 3.176939e-01 0.714838970 13 27425411 27426341 931 + 1.560 1.639 0.272
ENSG00000122034 E010 343.129085 2.878630e-04 1.359643e-01 0.519338182 13 27427092 27427192 101 + 2.551 2.523 -0.096
ENSG00000122034 E011 5.532853 2.950192e-02 3.841712e-01 0.763057368 13 27429172 27429212 41 + 0.734 0.869 0.535
ENSG00000122034 E012 9.171795 2.094551e-02 5.977490e-02 0.371024400 13 27429213 27429389 177 + 0.863 1.099 0.880
ENSG00000122034 E013 356.942662 1.780291e-04 3.640652e-01 0.749715760 13 27429870 27429966 97 + 2.562 2.547 -0.051
ENSG00000122034 E014 195.614221 1.443632e-03 4.586936e-01 0.807829267 13 27430533 27430537 5 + 2.302 2.281 -0.071
ENSG00000122034 E015 393.352699 6.678426e-04 5.494546e-01 0.853766843 13 27430538 27430621 84 + 2.602 2.591 -0.036
ENSG00000122034 E016 404.861773 1.497871e-04 2.687297e-01 0.673292215 13 27432731 27432804 74 + 2.598 2.619 0.070
ENSG00000122034 E017 417.347847 1.948104e-04 4.200805e-01 0.786131074 13 27434139 27434219 81 + 2.613 2.629 0.053
ENSG00000122034 E018 14.394571 3.621523e-03 5.208024e-05 0.009230333 13 27434657 27434804 148 + 0.945 1.327 1.373
ENSG00000122034 E019 249.053721 1.693117e-04 4.695659e-01 0.813688766 13 27434805 27434806 2 + 2.406 2.392 -0.047
ENSG00000122034 E020 808.052752 9.078766e-05 1.160914e-02 0.186372766 13 27434807 27435034 228 + 2.890 2.922 0.105
ENSG00000122034 E021 487.353016 1.714904e-03 2.598568e-02 0.262228658 13 27435133 27435192 60 + 2.660 2.712 0.174
ENSG00000122034 E022 567.378732 2.355092e-03 4.191324e-03 0.116124398 13 27435433 27435823 391 + 2.715 2.786 0.236