ENSG00000121578

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000359213 ENSG00000121578 HEK293_DMSO_6hA HEK293_OSMI2_6hA B4GALT4 protein_coding protein_coding 10.67208 4.674695 4.492564 1.266931 0.390518 -0.05720846 1.2503966 0.1718878 0.3458591 0.09585267 0.30731471 0.9682575 0.10307917 0.04556667 0.06800000 0.02243333 9.933520e-01 6.167692e-07 FALSE TRUE
ENST00000393765 ENSG00000121578 HEK293_DMSO_6hA HEK293_OSMI2_6hA B4GALT4 protein_coding protein_coding 10.67208 4.674695 4.492564 1.266931 0.390518 -0.05720846 6.8040252 2.6316297 3.0588428 1.08852261 0.56393469 0.2162665 0.63277917 0.53336667 0.68820000 0.15483333 7.436805e-01 6.167692e-07 FALSE TRUE
ENST00000467604 ENSG00000121578 HEK293_DMSO_6hA HEK293_OSMI2_6hA B4GALT4 protein_coding protein_coding 10.67208 4.674695 4.492564 1.266931 0.390518 -0.05720846 0.4023386 0.5634584 0.2725123 0.09682037 0.03681706 -1.0213753 0.04732500 0.12756667 0.06223333 -0.06533333 4.431343e-01 6.167692e-07 FALSE TRUE
ENST00000480814 ENSG00000121578 HEK293_DMSO_6hA HEK293_OSMI2_6hA B4GALT4 protein_coding nonsense_mediated_decay 10.67208 4.674695 4.492564 1.266931 0.390518 -0.05720846 0.8862160 0.9598703 0.0000000 0.25176199 0.00000000 -6.5997199 0.09937500 0.21653333 0.00000000 -0.21653333 6.167692e-07 6.167692e-07 TRUE TRUE
MSTRG.23486.7 ENSG00000121578 HEK293_DMSO_6hA HEK293_OSMI2_6hA B4GALT4 protein_coding   10.67208 4.674695 4.492564 1.266931 0.390518 -0.05720846 0.2573838 0.0000000 0.5776083 0.00000000 0.29518710 5.8767828 0.02692083 0.00000000 0.12736667 0.12736667 1.995391e-01 6.167692e-07 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000121578 E001 0.1125166 0.0326448178 0.4648397560   3 119211732 119211739 8 - 0.121 0.000 -9.533
ENSG00000121578 E002 0.0000000       3 119211740 119211741 2 -      
ENSG00000121578 E003 1.1522728 0.0170455876 0.5368956093   3 119211742 119211778 37 - 0.294 0.407 0.684
ENSG00000121578 E004 60.2817582 0.0005344706 0.0163809401 0.21685752 3 119211779 119212193 415 - 1.725 1.839 0.385
ENSG00000121578 E005 96.8714809 0.0003965053 0.0085063033 0.16074478 3 119212194 119212681 488 - 1.937 2.035 0.330
ENSG00000121578 E006 49.1534966 0.0240691422 0.0002204524 0.02164221 3 119212682 119216238 3557 - 1.498 1.817 1.086
ENSG00000121578 E007 1.1950136 0.0147646545 0.7774077336   3 119216239 119216239 1 - 0.295 0.351 0.352
ENSG00000121578 E008 37.3277967 0.0008731697 0.6436286036 0.89375243 3 119216240 119216344 105 - 1.596 1.579 -0.058
ENSG00000121578 E009 23.7924281 0.0013456930 0.4972228689 0.82796696 3 119218650 119218711 62 - 1.418 1.380 -0.134
ENSG00000121578 E010 29.2216287 0.0009734864 0.0834192128 0.42470549 3 119218712 119218772 61 - 1.531 1.433 -0.339
ENSG00000121578 E011 47.3069593 0.0006141052 0.0088439585 0.16396549 3 119224058 119224245 188 - 1.748 1.629 -0.402
ENSG00000121578 E012 27.7732358 0.0012081527 0.1677032936 0.56413699 3 119226809 119226885 77 - 1.506 1.427 -0.268
ENSG00000121578 E013 26.7458038 0.0012298284 0.1619577473 0.55669805 3 119226886 119226938 53 - 1.488 1.407 -0.279
ENSG00000121578 E014 24.0937626 0.0059661412 0.0278138107 0.27037343 3 119226939 119226988 50 - 1.479 1.325 -0.533
ENSG00000121578 E015 21.5548546 0.0015367606 0.1972238317 0.60053074 3 119226989 119227041 53 - 1.402 1.319 -0.288
ENSG00000121578 E016 28.0886816 0.0012189265 0.0740979019 0.40446809 3 119229847 119229935 89 - 1.526 1.422 -0.358
ENSG00000121578 E017 19.1666502 0.0016541937 0.3006660581 0.70048393 3 119229936 119229974 39 - 1.349 1.279 -0.244
ENSG00000121578 E018 23.8756600 0.0011242537 0.9825098866 0.99576558 3 119229975 119230034 60 - 1.391 1.402 0.037
ENSG00000121578 E019 23.6521375 0.0011970248 0.5329087112 0.84667934 3 119230035 119230079 45 - 1.408 1.374 -0.118
ENSG00000121578 E020 18.9012128 0.0016932527 0.6924110954 0.91252388 3 119230080 119230091 12 - 1.274 1.313 0.136
ENSG00000121578 E021 20.3048143 0.0017625311 0.6042562104 0.87739097 3 119230092 119230115 24 - 1.296 1.345 0.169
ENSG00000121578 E022 23.6840238 0.0011133413 0.7668174526 0.93841304 3 119230116 119230155 40 - 1.402 1.391 -0.039
ENSG00000121578 E023 19.2888404 0.0013817208 0.8393057311 0.95999923 3 119230156 119230175 20 - 1.296 1.320 0.083
ENSG00000121578 E024 26.9003950 0.0011847900 0.6641943839 0.90196341 3 119230176 119230244 69 - 1.429 1.466 0.127
ENSG00000121578 E025 0.0000000       3 119232417 119232521 105 -      
ENSG00000121578 E026 0.0000000       3 119233007 119233153 147 -      
ENSG00000121578 E027 1.8137247 0.2314911255 0.7594760822 0.93571410 3 119235145 119235227 83 - 0.531 0.408 -0.619
ENSG00000121578 E028 0.1767706 0.0361287657 0.4626532584   3 119236153 119236214 62 - 0.122 0.000 -12.024
ENSG00000121578 E029 0.1767706 0.0361287657 0.4626532584   3 119236215 119236251 37 - 0.122 0.000 -12.024
ENSG00000121578 E030 29.1668384 0.0029024269 0.7344006237 0.92727018 3 119236853 119237070 218 - 1.468 1.501 0.112
ENSG00000121578 E031 0.1544607 0.0314898870 0.5183702580   3 119237071 119237071 1 - 0.000 0.117 11.027
ENSG00000121578 E032 0.7065194 0.0939943713 0.3046607258   3 119240026 119240198 173 - 0.298 0.118 -1.669
ENSG00000121578 E033 0.4704972 0.0221893501 0.0763807374   3 119240199 119240315 117 - 0.295 0.000 -13.604
ENSG00000121578 E034 0.6422654 0.0193874923 0.2872934462   3 119240316 119240360 45 - 0.295 0.117 -1.645
ENSG00000121578 E035 1.6976919 0.0117360440 0.4185665722 0.78542213 3 119240361 119240530 170 - 0.360 0.502 0.753
ENSG00000121578 E036 0.7057227 0.0206618984 0.7069138516   3 119240531 119240564 34 - 0.216 0.286 0.531
ENSG00000121578 E037 0.9950099 0.0155075558 0.6541118376   3 119240565 119240664 100 - 0.360 0.286 -0.470
ENSG00000121578 E038 0.1125166 0.0326448178 0.4648397560   3 119240765 119240849 85 - 0.121 0.000 -12.041
ENSG00000121578 E039 9.7725338 0.0052831646 0.0769759663 0.41128671 3 119240850 119240986 137 - 1.132 0.957 -0.639