ENSG00000109475

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000394665 ENSG00000109475 HEK293_DMSO_6hA HEK293_OSMI2_6hA RPL34 protein_coding protein_coding 1035.293 536.7402 371.2118 91.78191 70.27735 -0.5319694 178.8915 103.2561 44.18094 18.54292 5.531774 -1.2245448 0.168025 0.1922 0.1228 -0.0694 0.002018599 0.002018599 FALSE  
ENST00000394667 ENSG00000109475 HEK293_DMSO_6hA HEK293_OSMI2_6hA RPL34 protein_coding protein_coding 1035.293 536.7402 371.2118 91.78191 70.27735 -0.5319694 833.7323 422.1663 321.35230 75.04293 64.641074 -0.3936448 0.810575 0.7842 0.8610 0.0768 0.003939965 0.002018599 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000109475 E001 1.5022104 1.237666e-02 3.262234e-01   4 108620482 108620539 58 + 0.258 0.437 1.093
ENSG00000109475 E002 18.2018270 1.557454e-03 4.180784e-02 0.31983008 4 108620540 108620568 29 + 1.133 1.313 0.634
ENSG00000109475 E003 18.6377702 1.435099e-03 6.591114e-02 0.38659841 4 108620569 108620575 7 + 1.159 1.318 0.562
ENSG00000109475 E004 417.1055982 1.321842e-04 7.382804e-02 0.40377472 4 108620576 108620589 14 + 2.616 2.586 -0.097
ENSG00000109475 E005 1127.8466616 6.197057e-04 2.248712e-03 0.08445871 4 108620590 108620600 11 + 3.060 3.012 -0.160
ENSG00000109475 E006 54.0721338 1.063900e-02 1.922674e-02 0.23175582 4 108620601 108620625 25 + 1.599 1.768 0.571
ENSG00000109475 E007 61.3651477 1.138629e-02 1.594386e-02 0.21457707 4 108620626 108620632 7 + 1.651 1.823 0.582
ENSG00000109475 E008 129.2143277 4.696450e-03 8.445241e-05 0.01231022 4 108620633 108620655 23 + 1.957 2.148 0.639
ENSG00000109475 E009 204.0157137 6.215337e-03 1.351277e-04 0.01619884 4 108620656 108620712 57 + 2.161 2.344 0.614
ENSG00000109475 E010 51.4608476 1.518150e-02 8.668064e-01 0.96735784 4 108620713 108621630 918 + 1.704 1.690 -0.047
ENSG00000109475 E011 5.2463931 4.588355e-03 9.884408e-01 0.99715438 4 108621631 108621696 66 + 0.769 0.774 0.020
ENSG00000109475 E012 23.4369828 6.342954e-03 8.388432e-01 0.95978971 4 108621697 108621950 254 + 1.354 1.371 0.057
ENSG00000109475 E013 2449.0576427 5.743079e-04 1.448644e-01 0.53199772 4 108621951 108622024 74 + 3.379 3.361 -0.061
ENSG00000109475 E014 6.7820336 2.002633e-02 2.690154e-01 0.67358112 4 108622043 108622104 62 + 0.769 0.924 0.602
ENSG00000109475 E015 3714.1935081 3.434512e-05 5.142740e-01 0.83726973 4 108622105 108622204 100 + 3.545 3.545 -0.001
ENSG00000109475 E016 14.6743759 2.586634e-03 5.152305e-01 0.83780392 4 108622205 108622514 310 + 1.206 1.149 -0.200
ENSG00000109475 E017 3547.1037538 4.324283e-04 5.057957e-01 0.83264260 4 108622515 108622618 104 + 3.523 3.530 0.024
ENSG00000109475 E018 26.8000569 6.285798e-02 6.783901e-01 0.90744619 4 108622619 108623332 714 + 1.397 1.434 0.127
ENSG00000109475 E019 2078.3869978 1.416311e-03 5.989344e-01 0.87514833 4 108625128 108625253 126 + 3.290 3.300 0.034
ENSG00000109475 E020 8.7625736 3.927130e-03 4.166771e-01 0.78458036 4 108625254 108625257 4 + 0.898 0.996 0.368
ENSG00000109475 E021 0.6825723 1.866378e-02 6.011779e-01   4 108625258 108625370 113 + 0.148 0.241 0.867
ENSG00000109475 E022 1.3617794 2.264469e-02 4.543146e-01   4 108625431 108625554 124 + 0.259 0.395 0.864
ENSG00000109475 E023 34.4391232 1.159630e-03 2.191163e-01 0.62373891 4 108630021 108630481 461 + 1.574 1.504 -0.241