ENSG00000105849

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000222567 ENSG00000105849 HEK293_DMSO_6hA HEK293_OSMI2_6hA POLR1F protein_coding protein_coding 15.68692 3.002105 3.311218 0.8900507 0.8021615 0.1409407 14.1900119 2.6903960 3.311218 0.9371986 0.8021615 0.2985415 0.9321333 0.8743333 1 0.1256667 0.0004035276 0.0004035276 FALSE TRUE
ENST00000462263 ENSG00000105849 HEK293_DMSO_6hA HEK293_OSMI2_6hA POLR1F protein_coding retained_intron 15.68692 3.002105 3.311218 0.8900507 0.8021615 0.1409407 0.8156421 0.3117085 0.000000 0.1034194 0.0000000 -5.0076823 0.0435875 0.1256667 0 -0.1256667 0.0128482058 0.0004035276   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000105849 E001 261.552769 0.002830932 0.001272695 0.06221149 7 19695461 19698564 3104 - 2.394 2.455 0.204
ENSG00000105849 E002 23.800019 0.001158214 0.015136322 0.20923607 7 19698565 19698727 163 - 1.470 1.319 -0.523
ENSG00000105849 E003 31.465537 0.001024491 0.397153560 0.77129067 7 19700072 19700273 202 - 1.540 1.505 -0.120
ENSG00000105849 E004 14.002739 0.002141135 0.398986055 0.77262859 7 19700274 19700280 7 - 1.211 1.153 -0.207
ENSG00000105849 E005 1.253469 0.012939521 0.015736780   7 19700281 19700335 55 - 0.110 0.536 3.080
ENSG00000105849 E006 29.032243 0.006772466 0.226767239 0.63171236 7 19704779 19704920 142 - 1.514 1.453 -0.209
ENSG00000105849 E007 26.962600 0.008861951 0.056904051 0.36343952 7 19708763 19709048 286 - 1.502 1.372 -0.448