ENSG00000100968

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000250373 ENSG00000100968 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFATC4 protein_coding protein_coding 1.917768 3.010893 3.089363 0.1093853 0.3935005 0.03699664 0.59979527 0.30338020 1.03494713 0.04054623 0.20441899 1.7374440 0.27298750 0.10193333 0.34340000 0.24146667 0.07943696 0.01499257 FALSE TRUE
ENST00000539237 ENSG00000100968 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFATC4 protein_coding protein_coding 1.917768 3.010893 3.089363 0.1093853 0.3935005 0.03699664 0.08145085 0.01752243 0.10676809 0.01752243 0.08009908 2.0849661 0.07859583 0.00570000 0.03253333 0.02683333 0.72434317 0.01499257 FALSE TRUE
ENST00000553708 ENSG00000100968 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFATC4 protein_coding protein_coding 1.917768 3.010893 3.089363 0.1093853 0.3935005 0.03699664 0.58724928 1.18029616 1.00260125 0.13398154 0.18006000 -0.2332544 0.27832500 0.39190000 0.33190000 -0.06000000 0.76237895 0.01499257 FALSE TRUE
ENST00000554655 ENSG00000100968 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFATC4 protein_coding retained_intron 1.917768 3.010893 3.089363 0.1093853 0.3935005 0.03699664 0.04117662 0.19579259 0.04775817 0.04480599 0.04775817 -1.8330940 0.01994167 0.06500000 0.01286667 -0.05213333 0.39843415 0.01499257 FALSE TRUE
ENST00000555393 ENSG00000100968 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFATC4 protein_coding protein_coding 1.917768 3.010893 3.089363 0.1093853 0.3935005 0.03699664 0.05959363 0.21625120 0.00000000 0.05279691 0.00000000 -4.4998535 0.03620000 0.07330000 0.00000000 -0.07330000 0.01499257 0.01499257 FALSE TRUE
ENST00000556302 ENSG00000100968 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFATC4 protein_coding retained_intron 1.917768 3.010893 3.089363 0.1093853 0.3935005 0.03699664 0.11421087 0.15225880 0.27202634 0.15225880 0.18651175 0.7975332 0.06531667 0.04963333 0.07603333 0.02640000 0.74723786 0.01499257 TRUE TRUE
ENST00000556957 ENSG00000100968 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFATC4 protein_coding retained_intron 1.917768 3.010893 3.089363 0.1093853 0.3935005 0.03699664 0.06045491 0.00000000 0.12979381 0.00000000 0.12979381 3.8052285 0.04033750 0.00000000 0.05503333 0.05503333 0.76893291 0.01499257 FALSE TRUE
ENST00000557028 ENSG00000100968 HEK293_DMSO_6hA HEK293_OSMI2_6hA NFATC4 protein_coding retained_intron 1.917768 3.010893 3.089363 0.1093853 0.3935005 0.03699664 0.21346012 0.78470094 0.39930140 0.13564327 0.21062236 -0.9572484 0.11503750 0.25893333 0.11456667 -0.14436667 0.60744650 0.01499257 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000100968 E001 0.0000000       14 24365673 24365718 46 +      
ENSG00000100968 E002 0.0000000       14 24366911 24366938 28 +      
ENSG00000100968 E003 0.1125166 0.0330371617 4.959206e-01   14 24366939 24367089 151 + 0.117 0.000 -11.628
ENSG00000100968 E004 0.1125166 0.0330371617 4.959206e-01   14 24367090 24367214 125 + 0.117 0.000 -14.051
ENSG00000100968 E005 0.0000000       14 24367260 24367351 92 +      
ENSG00000100968 E006 0.0000000       14 24367364 24367467 104 +      
ENSG00000100968 E007 0.1795728 0.0338133220 4.822175e-01   14 24367468 24367501 34 + 0.000 0.122 13.403
ENSG00000100968 E008 0.6938932 0.0294375897 1.913615e-01   14 24367502 24367653 152 + 0.349 0.122 -1.923
ENSG00000100968 E009 0.0000000       14 24368020 24368047 28 +      
ENSG00000100968 E010 0.1795728 0.0338133220 4.822175e-01   14 24368048 24368069 22 + 0.000 0.122 13.403
ENSG00000100968 E011 0.8838921 0.0218520550 1.650828e-01   14 24368070 24368169 100 + 0.117 0.362 2.067
ENSG00000100968 E012 1.0116034 0.0144971573 7.260193e-03   14 24368170 24368183 14 + 0.000 0.469 15.874
ENSG00000100968 E013 1.3679468 0.0123467914 2.365234e-02   14 24368184 24368185 2 + 0.117 0.515 2.874
ENSG00000100968 E014 12.8634238 0.0032610651 9.668493e-01 0.992408541 14 24368186 24368311 126 + 1.146 1.146 -0.001
ENSG00000100968 E015 12.3604244 0.0032906685 8.740615e-01 0.969535312 14 24368312 24368340 29 + 1.136 1.125 -0.039
ENSG00000100968 E016 17.4004588 0.0018196420 5.191422e-01 0.839742072 14 24368341 24368440 100 + 1.291 1.244 -0.166
ENSG00000100968 E017 0.0000000       14 24368999 24369081 83 +      
ENSG00000100968 E018 0.1544607 0.0362674426 4.817823e-01   14 24369082 24369152 71 + 0.000 0.122 13.392
ENSG00000100968 E019 0.1544607 0.0362674426 4.817823e-01   14 24369153 24369156 4 + 0.000 0.122 13.392
ENSG00000100968 E020 0.1544607 0.0362674426 4.817823e-01   14 24369157 24369166 10 + 0.000 0.122 13.392
ENSG00000100968 E021 0.4884942 0.0433603933 8.370376e-02   14 24369167 24369244 78 + 0.000 0.295 14.860
ENSG00000100968 E022 0.4884942 0.0433603933 8.370376e-02   14 24369245 24369343 99 + 0.000 0.295 14.860
ENSG00000100968 E023 0.1544607 0.0362674426 4.817823e-01   14 24369344 24369376 33 + 0.000 0.122 13.392
ENSG00000100968 E024 0.1717682 0.0335494213 4.823678e-01   14 24369377 24369498 122 + 0.000 0.122 13.404
ENSG00000100968 E025 15.9459089 0.0016955592 2.714121e-01 0.675468013 14 24369499 24369670 172 + 1.271 1.184 -0.306
ENSG00000100968 E026 66.9672357 0.0005820557 1.436587e-02 0.204814040 14 24369671 24370594 924 + 1.872 1.776 -0.323
ENSG00000100968 E027 1.2151843 0.3080869356 1.669584e-01   14 24371718 24371816 99 + 0.119 0.473 2.642
ENSG00000100968 E028 2.5383238 0.0082952510 1.789893e-01 0.577945159 14 24372078 24372440 363 + 0.407 0.628 1.066
ENSG00000100968 E029 36.3509194 0.0007776821 4.310885e-01 0.792526235 14 24372441 24372603 163 + 1.589 1.549 -0.136
ENSG00000100968 E030 0.1795728 0.0338133220 4.822175e-01   14 24372815 24373170 356 + 0.000 0.122 13.403
ENSG00000100968 E031 44.9358500 0.0058120954 8.472759e-01 0.962198378 14 24373171 24373370 200 + 1.660 1.655 -0.018
ENSG00000100968 E032 42.5095893 0.0007650796 6.416066e-01 0.893137564 14 24373695 24373867 173 + 1.641 1.622 -0.064
ENSG00000100968 E033 14.6169197 0.0380100669 3.478286e-01 0.737694723 14 24373868 24374122 255 + 1.126 1.231 0.375
ENSG00000100968 E034 11.5195292 0.0535069021 5.444477e-01 0.851894480 14 24374123 24374325 203 + 1.050 1.118 0.245
ENSG00000100968 E035 33.5801625 0.0010391352 3.655995e-02 0.302250717 14 24374326 24374466 141 + 1.589 1.471 -0.405
ENSG00000100968 E036 4.2016955 0.0087303512 6.613974e-02 0.387017580 14 24374467 24374707 241 + 0.542 0.814 1.153
ENSG00000100968 E037 23.0784014 0.0013011824 7.829675e-02 0.414191952 14 24375660 24375715 56 + 1.437 1.318 -0.412
ENSG00000100968 E038 29.0653519 0.0010633771 2.680936e-01 0.672610175 14 24375975 24376073 99 + 1.501 1.436 -0.223
ENSG00000100968 E039 17.0537009 0.0017326907 6.501163e-01 0.896458641 14 24376074 24376101 28 + 1.225 1.268 0.149
ENSG00000100968 E040 47.2971439 0.0008991734 2.357850e-01 0.640635155 14 24376294 24376554 261 + 1.711 1.657 -0.183
ENSG00000100968 E041 58.1168743 0.0118778286 5.823846e-01 0.867815719 14 24376555 24376878 324 + 1.790 1.756 -0.115
ENSG00000100968 E042 55.9144683 0.0006859285 3.162438e-05 0.006526083 14 24376879 24377637 759 + 1.635 1.832 0.665
ENSG00000100968 E043 79.5758775 0.0004909299 1.581536e-02 0.213833878 14 24377638 24379604 1967 + 1.853 1.948 0.318