ENSG00000100650

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000553369 ENSG00000100650 HEK293_DMSO_6hA HEK293_OSMI2_6hA SRSF5 protein_coding protein_coding 160.3875 150.9508 113.2107 46.29544 18.84729 -0.4150359 14.587919 7.786728 5.720776 4.434915 1.5690229 -0.44413829 0.08243750 0.04606667 0.04856667 0.002500000 0.88561464 0.04925368 FALSE FALSE
ENST00000554465 ENSG00000100650 HEK293_DMSO_6hA HEK293_OSMI2_6hA SRSF5 protein_coding retained_intron 160.3875 150.9508 113.2107 46.29544 18.84729 -0.4150359 10.697253 11.291282 5.248055 3.914051 1.9320446 -1.10388540 0.06340833 0.07323333 0.04353333 -0.029700000 0.23523724 0.04925368 FALSE FALSE
ENST00000556184 ENSG00000100650 HEK293_DMSO_6hA HEK293_OSMI2_6hA SRSF5 protein_coding retained_intron 160.3875 150.9508 113.2107 46.29544 18.84729 -0.4150359 16.243255 24.130476 15.168035 8.088990 4.0009614 -0.66946914 0.10289583 0.15803333 0.12953333 -0.028500000 0.65671751 0.04925368 FALSE TRUE
ENST00000556330 ENSG00000100650 HEK293_DMSO_6hA HEK293_OSMI2_6hA SRSF5 protein_coding retained_intron 160.3875 150.9508 113.2107 46.29544 18.84729 -0.4150359 8.064813 7.665942 5.853927 2.671074 0.2631351 -0.38847674 0.05114583 0.05070000 0.05420000 0.003500000 0.90362114 0.04925368 FALSE TRUE
ENST00000557154 ENSG00000100650 HEK293_DMSO_6hA HEK293_OSMI2_6hA SRSF5 protein_coding protein_coding 160.3875 150.9508 113.2107 46.29544 18.84729 -0.4150359 22.560478 22.132977 14.868727 7.236496 0.4787387 -0.57359808 0.15282083 0.14503333 0.13840000 -0.006633333 0.87396747 0.04925368 FALSE TRUE
MSTRG.9806.11 ENSG00000100650 HEK293_DMSO_6hA HEK293_OSMI2_6hA SRSF5 protein_coding   160.3875 150.9508 113.2107 46.29544 18.84729 -0.4150359 11.615273 21.043159 9.338048 5.547393 3.7514104 -1.17129963 0.07587500 0.14340000 0.07726667 -0.066133333 0.13880460 0.04925368 FALSE TRUE
MSTRG.9806.4 ENSG00000100650 HEK293_DMSO_6hA HEK293_OSMI2_6hA SRSF5 protein_coding   160.3875 150.9508 113.2107 46.29544 18.84729 -0.4150359 25.739262 10.584373 15.568597 2.889203 2.0998911 0.55626717 0.15831667 0.07173333 0.14320000 0.071466667 0.04925368 0.04925368 TRUE TRUE
MSTRG.9806.5 ENSG00000100650 HEK293_DMSO_6hA HEK293_OSMI2_6hA SRSF5 protein_coding   160.3875 150.9508 113.2107 46.29544 18.84729 -0.4150359 29.543934 27.126964 26.832959 6.904150 4.4365259 -0.01571565 0.18350417 0.18516667 0.23696667 0.051800000 0.15732587 0.04925368 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000100650 E001 0.2669773 0.0267728575 7.643845e-01   14 69726900 69727191 292 + 0.139 0.103 -0.497
ENSG00000100650 E002 0.2669773 0.0267728575 7.643845e-01   14 69766114 69766467 354 + 0.139 0.103 -0.497
ENSG00000100650 E003 0.1767706 0.0311067160 3.313596e-01   14 69766468 69766728 261 + 0.139 0.000 -9.621
ENSG00000100650 E004 1.3376447 0.0131924975 2.110437e-01   14 69766729 69767092 364 + 0.461 0.255 -1.240
ENSG00000100650 E005 2.6269442 0.0079786528 1.299817e-02 1.962027e-01 14 69767093 69767111 19 + 0.742 0.368 -1.764
ENSG00000100650 E006 3.1628604 0.0137009427 3.375345e-02 2.932880e-01 14 69767112 69767115 4 + 0.771 0.457 -1.395
ENSG00000100650 E007 30.2348418 0.0018003563 1.731774e-05 4.462994e-03 14 69767116 69767141 26 + 1.621 1.368 -0.867
ENSG00000100650 E008 46.7859856 0.0007297517 5.349538e-09 1.081057e-05 14 69767142 69767156 15 + 1.816 1.542 -0.931
ENSG00000100650 E009 333.4719696 0.0010755129 6.079314e-03 1.383787e-01 14 69767157 69767171 15 + 2.542 2.495 -0.157
ENSG00000100650 E010 373.5855820 0.0009314352 5.199498e-03 1.290964e-01 14 69767172 69767174 3 + 2.590 2.545 -0.150
ENSG00000100650 E011 391.2854904 0.0010807060 1.089640e-02 1.805992e-01 14 69767175 69767181 7 + 2.609 2.566 -0.143
ENSG00000100650 E012 414.1916155 0.0008821852 9.250485e-03 1.674194e-01 14 69767182 69767185 4 + 2.631 2.592 -0.131
ENSG00000100650 E013 638.9360837 0.0002543701 3.716266e-04 2.999524e-02 14 69767186 69767239 54 + 2.814 2.781 -0.112
ENSG00000100650 E014 492.8450054 0.0003654747 8.921919e-02 4.370164e-01 14 69767240 69767251 12 + 2.687 2.676 -0.038
ENSG00000100650 E015 319.2382079 0.0001400998 1.636195e-01 5.585979e-01 14 69767252 69767255 4 + 2.495 2.491 -0.015
ENSG00000100650 E016 109.5011495 0.0004188019 7.309032e-02 4.023428e-01 14 69767256 69767380 125 + 2.053 2.016 -0.125
ENSG00000100650 E017 3.8681639 0.0373007074 8.396622e-01 9.601793e-01 14 69767731 69767782 52 + 0.683 0.651 -0.136
ENSG00000100650 E018 2.1594830 0.0114352408 1.306242e-01 5.112899e-01 14 69767783 69767797 15 + 0.605 0.368 -1.181
ENSG00000100650 E019 7.3998604 0.0430441352 8.464546e-01 9.619589e-01 14 69767798 69767902 105 + 0.895 0.915 0.076
ENSG00000100650 E020 6.0672180 0.0438880615 6.852683e-01 9.100281e-01 14 69767903 69767939 37 + 0.800 0.854 0.212
ENSG00000100650 E021 7.2417318 0.0494589479 8.834466e-01 9.719829e-01 14 69767940 69767987 48 + 0.903 0.885 -0.067
ENSG00000100650 E022 6.8311974 0.1684762164 4.201318e-01 7.861650e-01 14 69767988 69768100 113 + 0.830 0.898 0.263
ENSG00000100650 E023 9.1864760 0.0199951151 6.977531e-02 3.951295e-01 14 69768101 69768137 37 + 0.828 1.066 0.893
ENSG00000100650 E024 1262.4831620 0.0001243036 3.954889e-05 7.558947e-03 14 69768138 69768282 145 + 3.103 3.080 -0.075
ENSG00000100650 E025 7.6338089 0.0331580158 7.927630e-02 4.161133e-01 14 69768283 69768603 321 + 0.744 1.004 0.998
ENSG00000100650 E026 576.2993704 0.0005010017 8.372168e-02 4.252926e-01 14 69768604 69768612 9 + 2.756 2.745 -0.035
ENSG00000100650 E027 841.6404735 0.0006090518 5.745910e-04 3.934500e-02 14 69768613 69768674 62 + 2.937 2.898 -0.128
ENSG00000100650 E028 7.9983796 0.0031678533 5.347756e-02 3.541556e-01 14 69768675 69768797 123 + 0.771 1.025 0.968
ENSG00000100650 E029 1039.9573029 0.0007284605 2.564576e-03 9.072871e-02 14 69768798 69768896 99 + 3.024 2.993 -0.104
ENSG00000100650 E030 80.6520019 0.0070837101 2.237517e-02 2.473014e-01 14 69768897 69769122 226 + 1.802 1.952 0.508
ENSG00000100650 E031 42.1459287 0.0064023259 9.458824e-02 4.473122e-01 14 69769123 69769181 59 + 1.538 1.664 0.428
ENSG00000100650 E032 774.9293030 0.0009781372 6.615623e-03 1.432656e-01 14 69769182 69769251 70 + 2.896 2.866 -0.102
ENSG00000100650 E033 473.1637315 0.0072224069 7.078419e-02 3.973030e-01 14 69769252 69769510 259 + 2.618 2.690 0.238
ENSG00000100650 E034 738.7143570 0.0091227236 8.255585e-03 1.587019e-01 14 69769511 69769745 235 + 2.792 2.894 0.339
ENSG00000100650 E035 953.2728494 0.0112536135 2.472830e-02 2.575300e-01 14 69769746 69770042 297 + 2.914 2.998 0.278
ENSG00000100650 E036 716.2085375 0.0048404748 1.570618e-04 1.761210e-02 14 69770043 69770447 405 + 2.761 2.888 0.424
ENSG00000100650 E037 322.5716925 0.0084959846 1.145546e-03 5.853449e-02 14 69770448 69770466 19 + 2.402 2.548 0.487
ENSG00000100650 E038 862.8681489 0.0009892949 3.108857e-01 7.087730e-01 14 69770467 69770540 74 + 2.899 2.941 0.139
ENSG00000100650 E039 23.7842850 0.0011346718 1.048910e-03 5.578159e-02 14 69770881 69770994 114 + 1.196 1.470 0.954
ENSG00000100650 E040 939.8411992 0.0006211755 6.043742e-01 8.773913e-01 14 69770995 69771105 111 + 2.944 2.974 0.100
ENSG00000100650 E041 2.5069833 0.0086277239 6.022009e-01 8.765821e-01 14 69771106 69771108 3 + 0.562 0.496 -0.312
ENSG00000100650 E042 374.4759782 0.0004321718 4.734519e-01 8.156788e-01 14 69771194 69771196 3 + 2.558 2.565 0.023
ENSG00000100650 E043 1442.1415693 0.0003357895 7.016841e-01 9.156839e-01 14 69771197 69771544 348 + 3.138 3.153 0.052
ENSG00000100650 E044 526.5155238 0.0003568780 1.374507e-03 6.490972e-02 14 69771545 69771916 372 + 2.659 2.740 0.272
ENSG00000100650 E045 163.4003935 0.0003025512 2.542571e-02 2.602390e-01 14 69771917 69772005 89 + 2.148 2.239 0.303