ENSG00000100523

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000357758 ENSG00000100523 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDHD1 protein_coding protein_coding 5.356767 1.766824 2.075717 0.6038813 0.3486164 0.2312426 1.3825879 0.00000000 0.1548809 0.00000000 0.15488086 4.043352 0.22542083 0.00000000 0.0564000 0.05640000 0.74957775 0.01210658 FALSE TRUE
ENST00000553406 ENSG00000100523 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDHD1 protein_coding retained_intron 5.356767 1.766824 2.075717 0.6038813 0.3486164 0.2312426 0.1320906 0.20447553 0.0000000 0.09000866 0.00000000 -4.422741 0.02551667 0.10803333 0.0000000 -0.10803333 0.01210658 0.01210658   FALSE
ENST00000612692 ENSG00000100523 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDHD1 protein_coding protein_coding 5.356767 1.766824 2.075717 0.6038813 0.3486164 0.2312426 0.3529728 0.45038278 0.0000000 0.26732062 0.00000000 -5.524762 0.09862500 0.21473333 0.0000000 -0.21473333 0.19557238 0.01210658 FALSE TRUE
ENST00000673822 ENSG00000100523 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDHD1 protein_coding protein_coding 5.356767 1.766824 2.075717 0.6038813 0.3486164 0.2312426 0.6832360 0.75542101 0.9987268 0.16680005 0.14137515 0.398210 0.18503333 0.50700000 0.4927333 -0.01426667 0.99219188 0.01210658 FALSE TRUE
MSTRG.9564.12 ENSG00000100523 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDHD1 protein_coding   5.356767 1.766824 2.075717 0.6038813 0.3486164 0.2312426 1.4526635 0.04018659 0.5391886 0.04018659 0.19492054 3.451928 0.23765000 0.03133333 0.2528000 0.22146667 0.20524145 0.01210658 FALSE TRUE
MSTRG.9564.9 ENSG00000100523 HEK293_DMSO_6hA HEK293_OSMI2_6hA DDHD1 protein_coding   5.356767 1.766824 2.075717 0.6038813 0.3486164 0.2312426 0.7983567 0.00000000 0.1837402 0.00000000 0.09540155 4.276051 0.12703333 0.00000000 0.0840000 0.08400000 0.31486798 0.01210658 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000100523 E001 0.3262289 0.027442404 2.145048e-01   14 53036745 53036754 10 - 0.000 0.227 9.867
ENSG00000100523 E002 277.4286116 0.016925538 9.838813e-05 0.013433134 14 53036755 53043967 7213 - 2.301 2.556 0.851
ENSG00000100523 E003 8.7199230 0.085632080 4.606756e-01 0.808924560 14 53043968 53044121 154 - 0.916 1.067 0.559
ENSG00000100523 E004 76.0757386 0.002038532 5.518828e-02 0.358932271 14 53044122 53046026 1905 - 1.808 1.973 0.553
ENSG00000100523 E005 4.8531438 0.011110273 1.980795e-01 0.601247940 14 53046027 53046197 171 - 0.626 0.875 1.010
ENSG00000100523 E006 11.6808398 0.002262812 8.764994e-01 0.970340358 14 53046198 53046432 235 - 1.062 1.157 0.340
ENSG00000100523 E007 6.5574972 0.029527592 9.842626e-01 0.996201987 14 53046433 53046447 15 - 0.839 0.912 0.280
ENSG00000100523 E008 14.8770377 0.002043611 4.796890e-01 0.819020664 14 53046448 53046602 155 - 1.195 1.215 0.072
ENSG00000100523 E009 13.2546617 0.062743286 3.044135e-01 0.703382755 14 53046603 53046684 82 - 1.177 1.139 -0.137
ENSG00000100523 E010 16.1254110 0.015659709 8.241291e-02 0.422712797 14 53046685 53046790 106 - 1.280 1.203 -0.271
ENSG00000100523 E011 21.0199943 0.002926810 3.176164e-01 0.714802717 14 53046791 53046949 159 - 1.354 1.360 0.020
ENSG00000100523 E012 2.5379993 0.008500117 6.803071e-01 0.908292073 14 53048548 53049033 486 - 0.485 0.611 0.587
ENSG00000100523 E013 11.5915312 0.002553005 5.525354e-01 0.855096109 14 53051844 53051927 84 - 1.104 1.125 0.076
ENSG00000100523 E014 0.7950889 0.017160230 6.867542e-01   14 53052510 53053006 497 - 0.200 0.307 0.811
ENSG00000100523 E015 4.6057643 0.028969573 5.377653e-01 0.848763736 14 53053007 53054437 1431 - 0.757 0.736 -0.085
ENSG00000100523 E016 24.5701942 0.007414128 9.573072e-01 0.990196475 14 53054438 53054629 192 - 1.380 1.457 0.268
ENSG00000100523 E017 25.1269223 0.006172398 2.856153e-02 0.273886084 14 53055660 53055912 253 - 1.469 1.385 -0.292
ENSG00000100523 E018 13.0485766 0.021070891 1.287769e-01 0.508349594 14 53058477 53058626 150 - 1.210 1.102 -0.386
ENSG00000100523 E019 10.4232670 0.004924927 3.655012e-01 0.750559516 14 53061126 53061201 76 - 1.082 1.065 -0.062
ENSG00000100523 E020 0.0000000       14 53061202 53061324 123 -      
ENSG00000100523 E021 17.1497672 0.005042961 1.271777e-01 0.506000477 14 53062943 53063141 199 - 1.299 1.250 -0.175
ENSG00000100523 E022 10.4071383 0.002541217 2.189355e-01 0.623573484 14 53063142 53063205 64 - 1.084 1.039 -0.164
ENSG00000100523 E023 12.5601573 0.029996756 3.273119e-02 0.290023326 14 53072597 53072703 107 - 1.222 1.025 -0.708
ENSG00000100523 E024 12.7495369 0.002290595 6.154067e-03 0.139068273 14 53073741 53073847 107 - 1.225 1.052 -0.621
ENSG00000100523 E025 16.1984817 0.001767445 3.474941e-02 0.296773214 14 53091785 53091910 126 - 1.289 1.197 -0.325
ENSG00000100523 E026 10.1928625 0.003073764 7.513959e-03 0.152461923 14 53091911 53091932 22 - 1.142 0.948 -0.709
ENSG00000100523 E027 0.0000000       14 53091933 53092472 540 -      
ENSG00000100523 E028 0.1125166 0.032475261 4.815759e-01   14 53092473 53092675 203 - 0.111 0.000 -11.784
ENSG00000100523 E029 0.0000000       14 53092676 53093315 640 -      
ENSG00000100523 E030 8.0712086 0.003390190 5.926277e-02 0.369631663 14 53093316 53093321 6 - 1.028 0.895 -0.496
ENSG00000100523 E031 12.8157302 0.008519806 3.643955e-03 0.108740518 14 53093322 53093444 123 - 1.239 1.025 -0.771
ENSG00000100523 E032 1.4302391 0.011884272 1.044254e-01   14 53093445 53093851 407 - 0.200 0.531 2.032
ENSG00000100523 E033 0.9734967 0.015378759 3.861584e-01   14 53096117 53096166 50 - 0.337 0.227 -0.777
ENSG00000100523 E034 0.9734967 0.015378759 3.861584e-01   14 53096167 53096218 52 - 0.337 0.227 -0.777
ENSG00000100523 E035 0.0000000       14 53103031 53103051 21 -      
ENSG00000100523 E036 0.0000000       14 53103299 53103682 384 -      
ENSG00000100523 E037 15.9238831 0.001906400 8.592307e-05 0.012391842 14 53103683 53103856 174 - 1.333 1.078 -0.905
ENSG00000100523 E038 0.0000000       14 53103857 53104146 290 -      
ENSG00000100523 E039 0.0000000       14 53149785 53150086 302 -      
ENSG00000100523 E040 9.2404939 0.021294514 1.482834e-02 0.207477428 14 53152261 53152318 58 - 1.099 0.875 -0.831
ENSG00000100523 E041 17.0326646 0.001544021 1.962086e-05 0.004831133 14 53152319 53152705 387 - 1.372 1.102 -0.953
ENSG00000100523 E042 8.4818601 0.007608970 1.802603e-03 0.075507469 14 53152706 53153323 618 - 1.112 0.834 -1.037