ENSG00000100519

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000445930 ENSG00000100519 HEK293_DMSO_6hA HEK293_OSMI2_6hA PSMC6 protein_coding protein_coding 78.57866 23.16288 20.81753 9.102742 1.083591 -0.1539458 10.78989 7.270943 3.449651 2.616905 1.7447074 -1.0734990 0.21960000 0.32316667 0.16436667 -0.158800000 7.495778e-01 6.120838e-13 FALSE TRUE
ENST00000556813 ENSG00000100519 HEK293_DMSO_6hA HEK293_OSMI2_6hA PSMC6 protein_coding protein_coding 78.57866 23.16288 20.81753 9.102742 1.083591 -0.1539458 12.28977 1.192883 1.235146 1.192883 1.2351457 0.0498181 0.10716667 0.02886667 0.05376667 0.024900000 1.000000e+00 6.120838e-13 FALSE FALSE
ENST00000612399 ENSG00000100519 HEK293_DMSO_6hA HEK293_OSMI2_6hA PSMC6 protein_coding protein_coding 78.57866 23.16288 20.81753 9.102742 1.083591 -0.1539458 10.05931 0.000000 2.758263 0.000000 0.9023973 8.1128371 0.09293333 0.00000000 0.13423333 0.134233333 6.120838e-13 6.120838e-13 FALSE TRUE
MSTRG.9547.4 ENSG00000100519 HEK293_DMSO_6hA HEK293_OSMI2_6hA PSMC6 protein_coding   78.57866 23.16288 20.81753 9.102742 1.083591 -0.1539458 38.16758 10.247690 8.991262 4.106850 0.3598667 -0.1885067 0.45917083 0.43993333 0.43250000 -0.007433333 9.416912e-01 6.120838e-13 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000100519 E001 0.2250332 0.1640849104 0.210559614   14 52707178 52707183 6 + 0.222 0.000 -12.471
ENSG00000100519 E002 0.9142451 0.0152082669 0.061159363   14 52707184 52707191 8 + 0.433 0.112 -2.542
ENSG00000100519 E003 3.0882771 0.0071470750 0.151066181 0.54082117 14 52707192 52707199 8 + 0.735 0.525 -0.917
ENSG00000100519 E004 3.5493325 0.0067647409 0.113400148 0.48154742 14 52707200 52707200 1 + 0.786 0.561 -0.953
ENSG00000100519 E005 3.8833660 0.0058237712 0.242157455 0.64718916 14 52707201 52707201 1 + 0.787 0.626 -0.664
ENSG00000100519 E006 6.8966232 0.0034916553 0.152067096 0.54219418 14 52707202 52707208 7 + 0.980 0.818 -0.617
ENSG00000100519 E007 19.0646482 0.0070132477 0.018204495 0.22700440 14 52707209 52707218 10 + 1.394 1.202 -0.671
ENSG00000100519 E008 20.6627816 0.0015378084 0.006206016 0.13953320 14 52707219 52707221 3 + 1.432 1.234 -0.689
ENSG00000100519 E009 40.3247107 0.0007892571 0.001044557 0.05563888 14 52707222 52707230 9 + 1.704 1.534 -0.581
ENSG00000100519 E010 89.6029388 0.0004205748 0.268980013 0.67355602 14 52707231 52707286 56 + 1.978 1.944 -0.114
ENSG00000100519 E011 92.5135532 0.0006670055 0.247309230 0.65276700 14 52707287 52707304 18 + 1.993 1.956 -0.123
ENSG00000100519 E012 2.2086471 0.1185975901 0.801407618 0.94873609 14 52707305 52707529 225 + 0.561 0.485 -0.360
ENSG00000100519 E013 126.6700952 0.0003245339 0.106019556 0.46826576 14 52708309 52708388 80 + 2.131 2.087 -0.146
ENSG00000100519 E014 106.4064216 0.0003463217 0.839992121 0.96027826 14 52708483 52708522 40 + 2.025 2.039 0.045
ENSG00000100519 E015 0.1767706 0.0370309978 0.434969797   14 52708523 52708706 184 + 0.128 0.000 -11.523
ENSG00000100519 E016 0.1544607 0.0326491905 0.552751260   14 52708707 52708763 57 + 0.000 0.112 10.913
ENSG00000100519 E017 111.1556427 0.0003673008 0.261181803 0.66643445 14 52708764 52708816 53 + 2.069 2.040 -0.099
ENSG00000100519 E018 1.9874268 0.0097756377 0.036830805 0.30324254 14 52708817 52709089 273 + 0.227 0.595 2.100
ENSG00000100519 E019 22.3526796 0.0187829764 0.956330623 0.98997000 14 52709575 52710335 761 + 1.369 1.360 -0.030
ENSG00000100519 E020 29.1114005 0.0761049520 0.094463371 0.44706205 14 52710546 52711100 555 + 1.350 1.551 0.695
ENSG00000100519 E021 127.4824860 0.0002609793 0.343920000 0.73493662 14 52711101 52711168 68 + 2.124 2.102 -0.074
ENSG00000100519 E022 155.4099030 0.0002327561 0.343266578 0.73441134 14 52711410 52711524 115 + 2.206 2.187 -0.063
ENSG00000100519 E023 108.9971890 0.0003950799 0.529835374 0.84484661 14 52713881 52713968 88 + 2.052 2.038 -0.046
ENSG00000100519 E024 0.1544607 0.0326491905 0.552751260   14 52713969 52714032 64 + 0.000 0.112 10.913
ENSG00000100519 E025 112.2001895 0.0003117260 0.782182449 0.94289559 14 52718081 52718142 62 + 2.058 2.056 -0.006
ENSG00000100519 E026 1.4966933 0.4634892434 0.889328080   14 52718143 52718228 86 + 0.394 0.386 -0.044
ENSG00000100519 E027 48.9365869 0.0006429146 0.553732246 0.85536492 14 52718229 52718230 2 + 1.680 1.717 0.125
ENSG00000100519 E028 56.1387305 0.0005331943 0.951489306 0.98870177 14 52718231 52718239 9 + 1.756 1.761 0.015
ENSG00000100519 E029 115.7612174 0.0024259684 0.908030835 0.97810087 14 52718240 52718352 113 + 2.069 2.071 0.005
ENSG00000100519 E030 1.0311111 0.1085381606 0.534280526   14 52718353 52718506 154 + 0.229 0.338 0.763
ENSG00000100519 E031 3.3401133 0.2014811129 0.624743933 0.88591335 14 52718507 52718976 470 + 0.582 0.658 0.334
ENSG00000100519 E032 112.6996583 0.0003040749 0.049248082 0.34226622 14 52718977 52719038 62 + 2.017 2.088 0.240
ENSG00000100519 E033 165.2103570 0.0002775637 0.216308622 0.62102968 14 52720861 52720981 121 + 2.199 2.239 0.134
ENSG00000100519 E034 159.3731880 0.0002286703 0.171704120 0.56919255 14 52721110 52721190 81 + 2.182 2.226 0.147
ENSG00000100519 E035 36.4493360 0.0825601557 0.169363508 0.56641017 14 52721500 52723744 2245 + 1.490 1.619 0.442
ENSG00000100519 E036 10.0800808 0.0186139680 0.199177780 0.60236802 14 52723745 52723874 130 + 0.950 1.095 0.533
ENSG00000100519 E037 5.2174403 0.0046174043 0.201242957 0.60483847 14 52723875 52723964 90 + 0.678 0.855 0.711
ENSG00000100519 E038 146.3596928 0.0023525051 0.215114325 0.61973293 14 52723965 52724036 72 + 2.143 2.189 0.152
ENSG00000100519 E039 174.4768361 0.0042089472 0.135431562 0.51846391 14 52727499 52728590 1092 + 2.212 2.264 0.175