ENSG00000100473

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000396618 ENSG00000100473 HEK293_DMSO_6hA HEK293_OSMI2_6hA COCH protein_coding protein_coding 27.87236 9.915257 11.07306 3.017453 1.496807 0.1591804 13.163724 4.6321940 4.71050624 1.1882005 1.06804966 0.02413476 0.48532083 0.47923333 0.413033333 -0.06620000 0.73572025 0.01896384 FALSE  
ENST00000553833 ENSG00000100473 HEK293_DMSO_6hA HEK293_OSMI2_6hA COCH protein_coding retained_intron 27.87236 9.915257 11.07306 3.017453 1.496807 0.1591804 0.848559 0.7331705 0.07841504 0.2777273 0.03970274 -3.07132949 0.02962083 0.07086667 0.007833333 -0.06303333 0.01896384 0.01896384 FALSE  
ENST00000644874 ENSG00000100473 HEK293_DMSO_6hA HEK293_OSMI2_6hA COCH protein_coding protein_coding 27.87236 9.915257 11.07306 3.017453 1.496807 0.1591804 6.916970 2.5405750 3.60601144 0.7709479 0.60097222 0.50357669 0.25516250 0.25670000 0.327233333 0.07053333 0.57212737 0.01896384 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000100473 E001 0.0000000       14 30874373 30874374 2 +      
ENSG00000100473 E002 1.1720143 0.0864918319 3.621593e-01   14 30874375 30874513 139 + 0.400 0.224 -1.160
ENSG00000100473 E003 1.1720143 0.0864918319 3.621593e-01   14 30874514 30874523 10 + 0.400 0.224 -1.160
ENSG00000100473 E004 7.0605754 0.0050697647 9.577067e-02 0.449704994 14 30874524 30874558 35 + 0.985 0.783 -0.773
ENSG00000100473 E005 8.1118862 0.0040873337 5.778517e-02 0.365536892 14 30874559 30874571 13 + 1.047 0.829 -0.820
ENSG00000100473 E006 24.9502979 0.0041181941 4.341112e-02 0.324740888 14 30874572 30874591 20 + 1.484 1.334 -0.518
ENSG00000100473 E007 22.2687395 0.0167529289 7.565650e-02 0.408263722 14 30874592 30874667 76 + 1.444 1.269 -0.610
ENSG00000100473 E008 23.1602548 0.0042253042 1.038065e-02 0.176499826 14 30874668 30874744 77 + 1.467 1.268 -0.690
ENSG00000100473 E009 16.4475944 0.0017634808 3.145047e-02 0.285162303 14 30874745 30874762 18 + 1.321 1.141 -0.637
ENSG00000100473 E010 10.6546569 0.0025734693 2.709443e-02 0.267392741 14 30874763 30874763 1 + 1.167 0.943 -0.818
ENSG00000100473 E011 26.1954856 0.0010702709 1.999271e-02 0.235963225 14 30874764 30874891 128 + 1.508 1.354 -0.533
ENSG00000100473 E012 17.0986334 0.0016100254 2.552184e-01 0.661008390 14 30874892 30874915 24 + 1.308 1.218 -0.317
ENSG00000100473 E013 31.4771924 0.0096451500 1.902346e-01 0.591794343 14 30874916 30874972 57 + 1.562 1.470 -0.316
ENSG00000100473 E014 0.8751032 0.0158946350 1.271774e-01   14 30874973 30875055 83 + 0.395 0.126 -2.137
ENSG00000100473 E015 28.8658575 0.0036755579 2.601467e-01 0.665683793 14 30875056 30875103 48 + 1.519 1.446 -0.251
ENSG00000100473 E016 3.6299462 0.0125398107 4.897263e-01 0.824464882 14 30875104 30875298 195 + 0.629 0.731 0.427
ENSG00000100473 E017 5.9697987 0.0496549843 9.585932e-01 0.990492531 14 30875590 30875916 327 + 0.846 0.829 -0.066
ENSG00000100473 E018 5.1196462 0.0046395865 2.922609e-05 0.006248529 14 30877336 30877571 236 + 0.396 0.990 2.558
ENSG00000100473 E019 23.6529873 0.0114769925 8.971018e-01 0.975474361 14 30877572 30877573 2 + 1.402 1.400 -0.006
ENSG00000100473 E020 30.1256418 0.0146168287 6.856291e-01 0.910198701 14 30877574 30877601 28 + 1.513 1.488 -0.084
ENSG00000100473 E021 56.8218705 0.0033815111 5.755826e-01 0.864661673 14 30877602 30877728 127 + 1.754 1.787 0.111
ENSG00000100473 E022 66.5506752 0.0004837695 2.498812e-01 0.655501078 14 30878811 30878944 134 + 1.807 1.856 0.167
ENSG00000100473 E023 59.1353910 0.0020396233 8.614801e-01 0.966210743 14 30879423 30879485 63 + 1.779 1.792 0.042
ENSG00000100473 E024 57.2306128 0.0006079303 7.287661e-01 0.925333557 14 30880452 30880496 45 + 1.764 1.781 0.056
ENSG00000100473 E025 83.3237204 0.0003687263 5.809658e-01 0.867344665 14 30880587 30880734 148 + 1.920 1.941 0.072
ENSG00000100473 E026 95.3756133 0.0010997600 9.217823e-01 0.981258649 14 30884553 30884656 104 + 1.986 1.990 0.015
ENSG00000100473 E027 6.2230371 0.0700734878 1.742906e-02 0.223116524 14 30884910 30884984 75 + 0.595 1.040 1.763
ENSG00000100473 E028 8.7462239 0.0195395444 1.341970e-02 0.199727492 14 30884985 30885041 57 + 0.804 1.127 1.207
ENSG00000100473 E029 159.0065955 0.0007584395 2.291222e-01 0.634273533 14 30885394 30885620 227 + 2.192 2.225 0.111
ENSG00000100473 E030 263.5127342 0.0006474257 3.232723e-01 0.719396344 14 30885796 30886312 517 + 2.415 2.436 0.067
ENSG00000100473 E031 117.3886835 0.0003795177 9.125667e-01 0.979097159 14 30889616 30889793 178 + 2.078 2.075 -0.012
ENSG00000100473 E032 156.5731594 0.0004878970 1.503468e-01 0.539820859 14 30889794 30890618 825 + 2.182 2.222 0.132
ENSG00000100473 E033 10.9411207 0.0228308985 8.791863e-01 0.970969690 14 30894913 30895567 655 + 1.083 1.070 -0.046