ENSG00000100139

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000215957 ENSG00000100139 HEK293_DMSO_6hA HEK293_OSMI2_6hA MICALL1 protein_coding protein_coding 31.63439 22.35111 50.71969 2.740677 10.76621 1.181838 18.316103 13.997465 28.50963 1.9189897 6.284201 1.025759 0.5659375 0.6236333 0.5594667 -0.06416667 0.212790050 0.001008333 FALSE TRUE
ENST00000681920 ENSG00000100139 HEK293_DMSO_6hA HEK293_OSMI2_6hA MICALL1 protein_coding nonsense_mediated_decay 31.63439 22.35111 50.71969 2.740677 10.76621 1.181838 8.636691 3.964711 14.72992 0.3155866 2.577274 1.890807 0.2767333 0.1798333 0.2968000 0.11696667 0.001008333 0.001008333 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000100139 E001 0.1717682 0.0327504191 2.558676e-01   22 37905657 37905726 70 + 0.000 0.159 9.500
ENSG00000100139 E002 2.9865648 0.0067093761 1.926119e-03 0.07799983 22 37906201 37906296 96 + 0.726 0.159 -3.290
ENSG00000100139 E003 3.1132627 0.0241102320 1.583792e-01 0.55180298 22 37906297 37906325 29 + 0.685 0.442 -1.123
ENSG00000100139 E004 20.3187422 0.0296276875 4.250991e-01 0.78932220 22 37906326 37906422 97 + 1.339 1.274 -0.227
ENSG00000100139 E005 112.5577287 0.0091186481 7.272376e-02 0.40153780 22 37906423 37906568 146 + 2.067 1.986 -0.272
ENSG00000100139 E006 10.2317763 0.0082967810 1.932550e-01 0.59549803 22 37906882 37907164 283 + 1.077 0.930 -0.543
ENSG00000100139 E007 13.8619809 0.0149675291 1.359360e-01 0.51929412 22 37907165 37907386 222 + 1.205 1.048 -0.565
ENSG00000100139 E008 10.4407672 0.0023104240 7.422470e-01 0.93008705 22 37907387 37907478 92 + 1.032 1.065 0.117
ENSG00000100139 E009 132.5580339 0.0060575403 1.528555e-01 0.54328411 22 37911952 37912000 49 + 2.126 2.074 -0.176
ENSG00000100139 E010 241.6465888 0.0011273982 1.344467e-01 0.51686030 22 37912351 37912492 142 + 2.382 2.344 -0.127
ENSG00000100139 E011 1.9285242 0.0489904100 9.243626e-01 0.98198999 22 37917137 37917706 570 + 0.451 0.443 -0.044
ENSG00000100139 E012 224.5343597 0.0008938388 9.932229e-01 0.99834530 22 37917707 37917795 89 + 2.336 2.338 0.008
ENSG00000100139 E013 242.0665491 0.0001369306 6.299477e-01 0.88803827 22 37919036 37919178 143 + 2.370 2.379 0.029
ENSG00000100139 E014 1.3952591 0.4015635311 2.554122e-01   22 37919179 37919655 477 + 0.236 0.510 1.627
ENSG00000100139 E015 308.4782201 0.0020551499 4.199544e-01 0.78602071 22 37921972 37922426 455 + 2.469 2.494 0.084
ENSG00000100139 E016 114.7344809 0.0018278361 2.912963e-01 0.69260513 22 37924660 37924717 58 + 2.034 2.078 0.146
ENSG00000100139 E017 365.3525871 0.0026229932 2.408036e-01 0.64579992 22 37925661 37926043 383 + 2.557 2.535 -0.075
ENSG00000100139 E018 4.3058742 0.0053570063 6.833006e-05 0.01088889 22 37926563 37926955 393 + 0.414 0.973 2.398
ENSG00000100139 E019 2.5094862 0.1961730743 5.642673e-01 0.85963192 22 37926956 37927126 171 + 0.483 0.612 0.598
ENSG00000100139 E020 551.6078470 0.0005034097 1.284939e-02 0.19525540 22 37927411 37927820 410 + 2.745 2.701 -0.147
ENSG00000100139 E021 138.5531442 0.0002407943 8.236373e-01 0.95539169 22 37927821 37927826 6 + 2.138 2.128 -0.033
ENSG00000100139 E022 353.2219421 0.0001299836 2.894865e-01 0.69106846 22 37931799 37931933 135 + 2.546 2.523 -0.077
ENSG00000100139 E023 362.0340053 0.0003216541 1.587429e-02 0.21408908 22 37932553 37932679 127 + 2.566 2.515 -0.170
ENSG00000100139 E024 303.9727992 0.0001705543 4.791760e-01 0.81877287 22 37932798 37932888 91 + 2.478 2.460 -0.059
ENSG00000100139 E025 311.0876017 0.0001289611 6.513657e-01 0.89712619 22 37933039 37933112 74 + 2.487 2.475 -0.040
ENSG00000100139 E026 178.7356460 0.0001889501 6.075162e-01 0.87862338 22 37937080 37937084 5 + 2.240 2.251 0.037
ENSG00000100139 E027 448.2054205 0.0001389480 3.322238e-01 0.72633297 22 37937085 37937194 110 + 2.636 2.650 0.047
ENSG00000100139 E028 370.4831239 0.0003857578 7.390041e-02 0.40394779 22 37937746 37937792 47 + 2.545 2.579 0.114
ENSG00000100139 E029 13.0012902 0.0504389667 7.786069e-02 0.41328698 22 37937793 37937819 27 + 1.027 1.261 0.841
ENSG00000100139 E030 1334.3513051 0.0008571019 4.558816e-01 0.80661743 22 37940709 37941533 825 + 3.105 3.121 0.053
ENSG00000100139 E031 397.7436800 0.0024607780 7.492076e-01 0.93242650 22 37941534 37941669 136 + 2.587 2.583 -0.014
ENSG00000100139 E032 765.1488905 0.0007660232 3.007592e-04 0.02646760 22 37941670 37942822 1153 + 2.842 2.911 0.227