ENSG00000099940

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000215730 ENSG00000099940 HEK293_DMSO_6hA HEK293_OSMI2_6hA SNAP29 protein_coding protein_coding 15.17232 10.29449 12.53606 0.9660097 2.438694 0.2839614 14.59084 9.281709 12.37135 0.780324 2.481633 0.4141534 0.958175 0.9032 0.9847333 0.08153333 0.003981257 0.003981257 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000099940 E001 1.4323519 0.0118767213 0.612114904   22 20858707 20858764 58 + 0.337 0.433 0.543
ENSG00000099940 E002 1.4806145 0.0692141774 0.817091111   22 20858765 20858799 35 + 0.388 0.433 0.248
ENSG00000099940 E003 0.5607039 0.0217681645 0.375771094   22 20858800 20858803 4 + 0.274 0.128 -1.362
ENSG00000099940 E004 38.2970883 0.0011123846 0.002189686 0.08335644 22 20859007 20859080 74 + 1.667 1.507 -0.547
ENSG00000099940 E005 40.4221684 0.0022642797 0.001658955 0.07224779 22 20859081 20859089 9 + 1.694 1.525 -0.575
ENSG00000099940 E006 181.3383752 0.0025160924 0.008603398 0.16189650 22 20859090 20859347 258 + 2.297 2.221 -0.251
ENSG00000099940 E007 0.1767706 0.0382417417 0.535668633   22 20859483 20859640 158 + 0.112 0.000 -9.179
ENSG00000099940 E008 82.0826458 0.0011606023 0.188543171 0.58996149 22 20870337 20870339 3 + 1.939 1.900 -0.133
ENSG00000099940 E009 220.9019237 0.0004385605 0.212798789 0.61739778 22 20870340 20870533 194 + 2.353 2.338 -0.053
ENSG00000099940 E010 1.8912944 0.0196351689 0.187681380 0.58884703 22 20870534 20871041 508 + 0.337 0.572 1.220
ENSG00000099940 E011 184.6416008 0.0002033162 0.713172873 0.91970783 22 20881049 20881134 86 + 2.254 2.278 0.078
ENSG00000099940 E012 222.8195760 0.0001983794 0.550796994 0.85430831 22 20883471 20883569 99 + 2.331 2.358 0.093
ENSG00000099940 E013 923.0160358 0.0023574082 0.023345958 0.25166766 22 20887679 20890542 2864 + 2.941 2.987 0.152
ENSG00000099940 E014 99.5129583 0.0028040462 0.810958906 0.95175095 22 20890543 20890860 318 + 1.988 2.009 0.070
ENSG00000099940 E015 80.7928467 0.0026836874 0.696667394 0.91382966 22 20890861 20891214 354 + 1.890 1.922 0.107