ENSG00000099849

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000397582 ENSG00000099849 HEK293_DMSO_6hA HEK293_OSMI2_6hA RASSF7 protein_coding protein_coding 27.23156 30.13189 39.01007 3.335732 5.944065 0.3724465 19.378987 18.105114 29.204312 2.3446613 5.0127084 0.6894814 0.7092125 0.5983 0.74350000 0.1452000 0.02330811 0.02330811 FALSE TRUE
MSTRG.4906.14 ENSG00000099849 HEK293_DMSO_6hA HEK293_OSMI2_6hA RASSF7 protein_coding   27.23156 30.13189 39.01007 3.335732 5.944065 0.3724465 3.046787 6.820863 2.631573 0.8961886 0.8718169 -1.3706704 0.1285333 0.2252 0.07383333 -0.1513667 0.05237451 0.02330811   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000099849 E001 1.3919549 0.5185735314 3.271011e-01   11 560404 560514 111 + 0.192 0.521 2.054
ENSG00000099849 E002 0.9104077 0.0150866297 2.100503e-01   11 560946 560955 10 + 0.184 0.401 1.527
ENSG00000099849 E003 1.6961447 0.0738591778 8.509328e-01 0.963210093 11 560956 560969 14 + 0.447 0.462 0.079
ENSG00000099849 E004 1.6961447 0.0738591778 8.509328e-01 0.963210093 11 560970 560970 1 + 0.447 0.462 0.079
ENSG00000099849 E005 15.3383142 0.0017092355 8.108995e-04 0.047887866 11 560971 560986 16 + 1.324 1.021 -1.080
ENSG00000099849 E006 85.1812725 0.0016425861 5.489131e-05 0.009570876 11 560987 561033 47 + 2.001 1.832 -0.568
ENSG00000099849 E007 11.0483701 0.0604388859 8.775661e-01 0.970616020 11 561034 561449 416 + 1.059 1.112 0.189
ENSG00000099849 E008 7.2003349 0.0224409157 4.450946e-01 0.800686690 11 561450 561477 28 + 0.862 0.973 0.420
ENSG00000099849 E009 10.2976360 0.1481756978 1.800685e-02 0.225885236 11 561547 561761 215 + 0.773 1.250 1.769
ENSG00000099849 E010 184.8106144 0.0002624124 8.154169e-03 0.157705321 11 561762 561892 131 + 2.295 2.228 -0.224
ENSG00000099849 E011 116.9504906 0.0003339596 1.696425e-01 0.566589336 11 562079 562083 5 + 2.086 2.043 -0.145
ENSG00000099849 E012 395.9552580 0.0002806829 6.947367e-02 0.394422761 11 562084 562384 301 + 2.608 2.579 -0.096
ENSG00000099849 E013 404.5914111 0.0001334219 1.995486e-01 0.602826759 11 562385 562776 392 + 2.617 2.596 -0.068
ENSG00000099849 E014 5.8761760 0.0070046598 5.218801e-03 0.129349858 11 562910 562948 39 + 0.621 0.989 1.459
ENSG00000099849 E015 10.5443876 0.0024286100 5.013872e-04 0.036123640 11 563035 563188 154 + 0.866 1.220 1.296
ENSG00000099849 E016 197.2746471 0.0021098414 7.104323e-01 0.918907386 11 563189 563317 129 + 2.303 2.291 -0.041
ENSG00000099849 E017 113.8594596 0.0002936487 7.563736e-01 0.934650556 11 563318 563347 30 + 2.054 2.065 0.036
ENSG00000099849 E018 131.7177066 0.0004005312 7.892054e-01 0.944756228 11 563348 563395 48 + 2.119 2.128 0.031
ENSG00000099849 E019 193.1227538 0.0002486629 2.044150e-01 0.608235603 11 563396 563478 83 + 2.274 2.306 0.108
ENSG00000099849 E020 200.4021456 0.0002819362 6.736332e-03 0.144673343 11 563479 563557 79 + 2.269 2.338 0.229
ENSG00000099849 E021 497.6093244 0.0005492851 2.724347e-03 0.093681030 11 563558 564025 468 + 2.671 2.725 0.181