ENSG00000086232

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000199389 ENSG00000086232 HEK293_DMSO_6hA HEK293_OSMI2_6hA EIF2AK1 protein_coding protein_coding 88.1136 56.21377 83.86773 6.460679 16.43294 0.5771076 22.32217 16.167823 21.76961 1.8640286 4.288854 0.4289605 0.2674125 0.2876667 0.2601667 -0.02750000 0.57701405 0.01698399 FALSE TRUE
MSTRG.29457.1 ENSG00000086232 HEK293_DMSO_6hA HEK293_OSMI2_6hA EIF2AK1 protein_coding   88.1136 56.21377 83.86773 6.460679 16.43294 0.5771076 13.33299 9.870056 14.03222 1.5171999 3.631675 0.5071795 0.1592792 0.1769667 0.1634333 -0.01353333 0.89217497 0.01698399 TRUE TRUE
MSTRG.29457.4 ENSG00000086232 HEK293_DMSO_6hA HEK293_OSMI2_6hA EIF2AK1 protein_coding   88.1136 56.21377 83.86773 6.460679 16.43294 0.5771076 22.03397 6.814945 16.78094 0.9063646 3.231366 1.2987935 0.2228208 0.1220333 0.2010667 0.07903333 0.01698399 0.01698399 FALSE TRUE
MSTRG.29457.5 ENSG00000086232 HEK293_DMSO_6hA HEK293_OSMI2_6hA EIF2AK1 protein_coding   88.1136 56.21377 83.86773 6.460679 16.43294 0.5771076 27.88702 19.465360 28.07658 3.3020020 6.373867 0.5282311 0.3180667 0.3423667 0.3296000 -0.01276667 0.86127083 0.01698399 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000086232 E001 18.5670971 1.552208e-03 6.249120e-04 4.150443e-02 7 6018905 6019313 409 - 1.140 1.416 0.967
ENSG00000086232 E002 98.9587496 1.936156e-03 1.012369e-02 1.744674e-01 7 6022247 6022786 540 - 1.942 2.053 0.373
ENSG00000086232 E003 99.0336205 3.704134e-04 1.090631e-04 1.417469e-02 7 6022787 6023175 389 - 1.929 2.071 0.475
ENSG00000086232 E004 136.0785200 9.387609e-04 4.272799e-03 1.171866e-01 7 6023176 6023721 546 - 2.086 2.186 0.334
ENSG00000086232 E005 29.8569036 8.910792e-04 1.877759e-01 5.888702e-01 7 6023722 6023746 25 - 1.447 1.533 0.297
ENSG00000086232 E006 1417.9572109 2.080319e-03 7.836502e-02 4.143387e-01 7 6023747 6024284 538 - 3.131 3.173 0.142
ENSG00000086232 E007 651.5746227 3.413598e-04 8.681840e-01 9.675765e-01 7 6024285 6024355 71 - 2.815 2.815 -0.001
ENSG00000086232 E008 1171.9378968 2.031473e-04 7.781384e-02 4.132138e-01 7 6024356 6024708 353 - 3.058 3.082 0.079
ENSG00000086232 E009 535.1093599 9.661873e-05 2.754566e-01 6.791393e-01 7 6024709 6024801 93 - 2.718 2.739 0.068
ENSG00000086232 E010 12.7253924 2.109548e-03 1.599922e-08 2.364384e-05 7 6026435 6026727 293 - 0.791 1.350 2.043
ENSG00000086232 E011 925.4689004 5.220971e-04 7.980288e-01 9.476433e-01 7 6026728 6026961 234 - 2.962 2.969 0.022
ENSG00000086232 E012 647.8976513 7.387396e-05 1.374738e-01 5.214155e-01 7 6028615 6028697 83 - 2.819 2.802 -0.058
ENSG00000086232 E013 2.0905015 1.012979e-02 2.237708e-03 8.422402e-02 7 6028698 6028839 142 - 0.189 0.703 2.893
ENSG00000086232 E014 689.8039495 8.229540e-05 2.403852e-01 6.455030e-01 7 6028918 6029032 115 - 2.844 2.831 -0.042
ENSG00000086232 E015 575.1713665 7.604167e-04 3.588534e-01 7.459905e-01 7 6037424 6037524 101 - 2.766 2.750 -0.053
ENSG00000086232 E016 616.9879993 8.635117e-05 2.221369e-01 6.267945e-01 7 6038560 6038671 112 - 2.797 2.782 -0.048
ENSG00000086232 E017 529.6906638 8.608433e-05 6.242496e-01 8.857550e-01 7 6040892 6040958 67 - 2.719 2.730 0.036
ENSG00000086232 E018 376.0728241 1.070022e-03 4.155654e-01 7.838010e-01 7 6040959 6040975 17 - 2.566 2.589 0.076
ENSG00000086232 E019 770.4988895 7.699144e-05 9.166223e-01 9.800314e-01 7 6040976 6041152 177 - 2.886 2.888 0.007
ENSG00000086232 E020 416.4210075 1.151977e-04 7.139201e-02 3.984998e-01 7 6041153 6041219 67 - 2.633 2.604 -0.094
ENSG00000086232 E021 394.3017598 1.062215e-04 4.348836e-03 1.182931e-01 7 6042933 6042990 58 - 2.617 2.569 -0.160
ENSG00000086232 E022 220.9770676 1.521463e-04 2.012200e-02 2.364652e-01 7 6042991 6042993 3 - 2.368 2.316 -0.175
ENSG00000086232 E023 497.7666029 9.029091e-05 2.496109e-02 2.584527e-01 7 6044562 6044661 100 - 2.710 2.678 -0.108
ENSG00000086232 E024 476.6745684 6.902268e-04 5.603016e-01 8.581531e-01 7 6046071 6046151 81 - 2.681 2.674 -0.024
ENSG00000086232 E025 1.7641461 1.429715e-01 8.983264e-01 9.757075e-01 7 6046152 6046437 286 - 0.424 0.453 0.154
ENSG00000086232 E026 4.5216892 4.058145e-02 7.055932e-01 9.171522e-01 7 6046723 6046776 54 - 0.754 0.704 -0.201
ENSG00000086232 E027 25.9705317 5.592541e-02 6.334526e-01 8.893703e-01 7 6046777 6046991 215 - 1.393 1.463 0.240
ENSG00000086232 E028 505.6397013 4.001271e-03 8.365917e-01 9.591038e-01 7 6046992 6047091 100 - 2.703 2.703 0.000
ENSG00000086232 E029 0.4465501 2.176816e-02 4.122581e-01   7 6047204 6047246 43 - 0.105 0.240 1.437
ENSG00000086232 E030 305.8703980 5.308407e-03 6.380515e-01 8.913655e-01 7 6048807 6048844 38 - 2.490 2.477 -0.044
ENSG00000086232 E031 0.8280057 3.456923e-01 6.555739e-01   7 6049784 6049911 128 - 0.188 0.327 1.054
ENSG00000086232 E032 553.5109296 2.801089e-03 9.031901e-01 9.770406e-01 7 6049912 6050045 134 - 2.738 2.746 0.028
ENSG00000086232 E033 486.1070199 9.775880e-04 2.150571e-01 6.196870e-01 7 6054546 6054704 159 - 2.696 2.673 -0.076
ENSG00000086232 E034 0.8415334 3.467352e-01 4.120194e-02   7 6058148 6058242 95 - 0.000 0.454 11.141
ENSG00000086232 E035 253.7041325 8.265836e-04 7.656203e-04 4.650008e-02 7 6058966 6059201 236 - 2.443 2.358 -0.283