ENSG00000077463

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000337491 ENSG00000077463 HEK293_DMSO_6hA HEK293_OSMI2_6hA SIRT6 protein_coding protein_coding 35.80876 36.67781 52.99919 5.363952 8.440088 0.5309418 18.674499 18.4802886 30.602818 2.5192920 6.0712300 0.7273681 0.49122500 0.50636667 0.56880000 0.062433333 0.44621400 0.01958824 FALSE TRUE
ENST00000594341 ENSG00000077463 HEK293_DMSO_6hA HEK293_OSMI2_6hA SIRT6 protein_coding retained_intron 35.80876 36.67781 52.99919 5.363952 8.440088 0.5309418 2.272812 2.4590189 2.314538 1.4668826 1.1586934 -0.0869936 0.07330000 0.05890000 0.05156667 -0.007333333 1.00000000 0.01958824 FALSE FALSE
ENST00000596298 ENSG00000077463 HEK293_DMSO_6hA HEK293_OSMI2_6hA SIRT6 protein_coding retained_intron 35.80876 36.67781 52.99919 5.363952 8.440088 0.5309418 1.817238 0.5592412 2.289792 0.5592412 0.6974201 2.0143911 0.05178750 0.01310000 0.04130000 0.028200000 0.27504014 0.01958824 FALSE TRUE
ENST00000599394 ENSG00000077463 HEK293_DMSO_6hA HEK293_OSMI2_6hA SIRT6 protein_coding retained_intron 35.80876 36.67781 52.99919 5.363952 8.440088 0.5309418 2.443168 4.6818524 1.734394 0.2140286 0.4515134 -1.4274318 0.08601250 0.13233333 0.03736667 -0.094966667 0.01958824 0.01958824 FALSE FALSE
ENST00000601069 ENSG00000077463 HEK293_DMSO_6hA HEK293_OSMI2_6hA SIRT6 protein_coding retained_intron 35.80876 36.67781 52.99919 5.363952 8.440088 0.5309418 1.848530 2.7082893 2.166515 0.6121126 0.2369719 -0.3206789 0.06225833 0.07410000 0.04173333 -0.032366667 0.27206292 0.01958824 TRUE TRUE
ENST00000601571 ENSG00000077463 HEK293_DMSO_6hA HEK293_OSMI2_6hA SIRT6 protein_coding protein_coding 35.80876 36.67781 52.99919 5.363952 8.440088 0.5309418 3.100127 2.6064908 4.941284 0.7411100 0.9251132 0.9201696 0.08395417 0.06753333 0.09263333 0.025100000 0.58547951 0.01958824 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000077463 E001 0.1795728 0.0324438222 0.346225574   19 4174109 4174109 1 - 0.000 0.142 8.957
ENSG00000077463 E002 23.2672168 0.0012011701 0.126588451 0.50504407 19 4174110 4174140 31 - 1.426 1.325 -0.350
ENSG00000077463 E003 333.7548219 0.0001737672 0.615482895 0.88235055 19 4174141 4174335 195 - 2.515 2.535 0.068
ENSG00000077463 E004 481.4985759 0.0002986000 0.530164139 0.84504041 19 4174336 4174619 284 - 2.673 2.695 0.075
ENSG00000077463 E005 107.1513012 0.0003812424 0.996152236 0.99898494 19 4174620 4174635 16 - 2.030 2.041 0.036
ENSG00000077463 E006 133.3324014 0.0037637963 0.820163079 0.95459165 19 4174636 4174703 68 - 2.117 2.140 0.078
ENSG00000077463 E007 118.5136058 0.0099812584 0.754826379 0.93411532 19 4174704 4174860 157 - 2.060 2.092 0.109
ENSG00000077463 E008 40.7303693 0.0012640930 0.647264771 0.89538079 19 4174861 4174877 17 - 1.628 1.612 -0.054
ENSG00000077463 E009 144.5924522 0.0009961706 0.239291650 0.64453128 19 4174878 4174946 69 - 2.170 2.144 -0.084
ENSG00000077463 E010 28.4724401 0.0010819347 0.632337971 0.88888360 19 4174947 4175027 81 - 1.447 1.489 0.146
ENSG00000077463 E011 193.0934658 0.0002289006 0.055356811 0.35926018 19 4175028 4175111 84 - 2.302 2.263 -0.127
ENSG00000077463 E012 173.9316097 0.0004829373 0.064109650 0.38194879 19 4175112 4175151 40 - 2.257 2.218 -0.131
ENSG00000077463 E013 93.3435727 0.0319854790 0.012428221 0.19241422 19 4175152 4175571 420 - 1.842 2.095 0.848
ENSG00000077463 E014 42.5099900 0.0311846202 0.023142335 0.25064137 19 4175572 4175679 108 - 1.506 1.755 0.848
ENSG00000077463 E015 263.1799511 0.0028884783 0.198708329 0.60178296 19 4175680 4175760 81 - 2.424 2.409 -0.050
ENSG00000077463 E016 16.8534447 0.0550132060 0.272666047 0.67657864 19 4175761 4175790 30 - 1.147 1.339 0.676
ENSG00000077463 E017 19.8419973 0.0075918897 0.013414945 0.19969517 19 4175791 4175841 51 - 1.199 1.425 0.790
ENSG00000077463 E018 302.6927004 0.0001446228 0.744086284 0.93078330 19 4175842 4175937 96 - 2.480 2.484 0.014
ENSG00000077463 E019 241.1798547 0.0001633085 0.929021757 0.98308151 19 4177079 4177138 60 - 2.378 2.391 0.042
ENSG00000077463 E020 346.4837385 0.0001259405 0.006730002 0.14464615 19 4179104 4179286 183 - 2.557 2.517 -0.133
ENSG00000077463 E021 31.8645565 0.1315029596 0.002576392 0.09093319 19 4179287 4179503 217 - 1.195 1.736 1.865
ENSG00000077463 E022 20.8013694 0.1226299458 0.225210882 0.63025805 19 4180580 4180611 32 - 1.230 1.440 0.732
ENSG00000077463 E023 37.8543764 0.0672127851 0.230443454 0.63538177 19 4180612 4180781 170 - 1.503 1.671 0.574
ENSG00000077463 E024 274.6507316 0.0018002281 0.289031237 0.69062837 19 4180782 4180909 128 - 2.444 2.430 -0.046
ENSG00000077463 E025 8.0052079 0.0241866272 0.552270144 0.85499381 19 4182211 4182473 263 - 0.961 0.903 -0.219
ENSG00000077463 E026 187.3117970 0.0003877483 0.003997893 0.11318770 19 4182474 4182566 93 - 2.301 2.235 -0.220