ENSG00000077092

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000330688 ENSG00000077092 HEK293_DMSO_6hA HEK293_OSMI2_6hA RARB protein_coding protein_coding 2.135156 1.046625 1.040627 0.3751926 0.2286782 -0.008214095 1.17329402 0.20260903 0.71731251 0.10502178 0.26121184 1.774373 0.47195417 0.31146667 0.63673333 0.32526667 0.644759067 0.002018599 FALSE TRUE
ENST00000437042 ENSG00000077092 HEK293_DMSO_6hA HEK293_OSMI2_6hA RARB protein_coding protein_coding 2.135156 1.046625 1.040627 0.3751926 0.2286782 -0.008214095 0.03340544 0.07662740 0.00000000 0.07662740 0.00000000 -3.114823 0.02311250 0.13030000 0.00000000 -0.13030000 0.795025656 0.002018599 FALSE TRUE
ENST00000458646 ENSG00000077092 HEK293_DMSO_6hA HEK293_OSMI2_6hA RARB protein_coding protein_coding 2.135156 1.046625 1.040627 0.3751926 0.2286782 -0.008214095 0.25803420 0.30094928 0.00000000 0.30094928 0.00000000 -4.958607 0.11349167 0.16810000 0.00000000 -0.16810000 0.749698894 0.002018599 FALSE TRUE
ENST00000479097 ENSG00000077092 HEK293_DMSO_6hA HEK293_OSMI2_6hA RARB protein_coding nonsense_mediated_decay 2.135156 1.046625 1.040627 0.3751926 0.2286782 -0.008214095 0.08551376 0.18367199 0.00000000 0.18367199 0.00000000 -4.275543 0.05426667 0.10260000 0.00000000 -0.10260000 0.747961452 0.002018599 FALSE TRUE
ENST00000480001 ENSG00000077092 HEK293_DMSO_6hA HEK293_OSMI2_6hA RARB protein_coding nonsense_mediated_decay 2.135156 1.046625 1.040627 0.3751926 0.2286782 -0.008214095 0.02853614 0.10740583 0.00000000 0.10740583 0.00000000 -3.553432 0.01348750 0.06000000 0.00000000 -0.06000000 0.744363154 0.002018599 TRUE TRUE
ENST00000685523 ENSG00000077092 HEK293_DMSO_6hA HEK293_OSMI2_6hA RARB protein_coding nonsense_mediated_decay 2.135156 1.046625 1.040627 0.3751926 0.2286782 -0.008214095 0.05305611 0.04475097 0.00000000 0.04475097 0.00000000 -2.452885 0.02242500 0.05876667 0.00000000 -0.05876667 0.753337631 0.002018599 FALSE TRUE
ENST00000687083 ENSG00000077092 HEK293_DMSO_6hA HEK293_OSMI2_6hA RARB protein_coding protein_coding 2.135156 1.046625 1.040627 0.3751926 0.2286782 -0.008214095 0.01363568 0.00000000 0.04065333 0.00000000 0.04065333 2.340657 0.01045833 0.00000000 0.06306667 0.06306667 0.750883821 0.002018599 FALSE TRUE
ENST00000688892 ENSG00000077092 HEK293_DMSO_6hA HEK293_OSMI2_6hA RARB protein_coding protein_coding 2.135156 1.046625 1.040627 0.3751926 0.2286782 -0.008214095 0.21713564 0.00000000 0.26105865 0.00000000 0.01076220 4.760533 0.12790417 0.00000000 0.28003333 0.28003333 0.002018599 0.002018599 FALSE TRUE
ENST00000689700 ENSG00000077092 HEK293_DMSO_6hA HEK293_OSMI2_6hA RARB protein_coding nonsense_mediated_decay 2.135156 1.046625 1.040627 0.3751926 0.2286782 -0.008214095 0.04020462 0.10731374 0.00000000 0.10731374 0.00000000 -3.552300 0.04595000 0.14093333 0.00000000 -0.14093333 0.774231047 0.002018599 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000077092 E001 0.0000000       3 24687887 24687900 14 +      
ENSG00000077092 E002 0.0000000       3 24687901 24687918 18 +      
ENSG00000077092 E003 0.0000000       3 24687919 24687995 77 +      
ENSG00000077092 E004 0.1767706 0.041894015 5.135031e-01   3 24762240 24762294 55 + 0.120 0.000 -10.699
ENSG00000077092 E005 0.0000000       3 24829323 24829336 14 +      
ENSG00000077092 E006 0.0000000       3 24829337 24829339 3 +      
ENSG00000077092 E007 0.0000000       3 24829340 24829403 64 +      
ENSG00000077092 E008 0.0000000       3 24833089 24833222 134 +      
ENSG00000077092 E009 0.3624201 0.249132472 2.812560e-01   3 24858674 24858752 79 + 0.219 0.000 -13.309
ENSG00000077092 E010 0.5391907 0.028526908 9.387315e-02   3 25060125 25060176 52 + 0.291 0.000 -14.114
ENSG00000077092 E011 0.1717682 0.035827150 4.636272e-01   3 25076163 25076283 121 + 0.000 0.120 11.659
ENSG00000077092 E012 0.0000000       3 25120578 25120621 44 +      
ENSG00000077092 E013 0.0000000       3 25132161 25132208 48 +      
ENSG00000077092 E014 2.1070221 0.009425291 3.156496e-05 0.006526083 3 25174119 25174575 457 + 0.735 0.000 -15.746
ENSG00000077092 E015 0.0000000       3 25427886 25428049 164 +      
ENSG00000077092 E016 0.0000000       3 25428096 25428136 41 +      
ENSG00000077092 E017 0.1767706 0.041894015 5.135031e-01   3 25428137 25428195 59 + 0.120 0.000 -12.709
ENSG00000077092 E018 0.3485388 0.027888884 9.811456e-01   3 25428196 25428262 67 + 0.120 0.120 0.001
ENSG00000077092 E019 1.2856958 0.032869938 9.303307e-01   3 25428263 25428401 139 + 0.355 0.356 0.008
ENSG00000077092 E020 2.2216879 0.024473282 3.591313e-01 0.746269830 3 25428402 25428531 130 + 0.585 0.413 -0.842
ENSG00000077092 E021 3.2715310 0.009826194 8.551375e-03 0.161247133 3 25428532 25428700 169 + 0.785 0.356 -2.004
ENSG00000077092 E022 2.5073072 0.011493783 8.456813e-02 0.426921586 3 25428701 25428715 15 + 0.653 0.356 -1.464
ENSG00000077092 E023 5.7318096 0.004331272 6.053393e-02 0.372815201 3 25428716 25428888 173 + 0.935 0.682 -0.998
ENSG00000077092 E024 0.0000000       3 25428889 25428892 4 +      
ENSG00000077092 E025 0.0000000       3 25451626 25451662 37 +      
ENSG00000077092 E026 0.0000000       3 25458310 25458393 84 +      
ENSG00000077092 E027 5.0430705 0.009640151 4.597644e-01 0.808428523 3 25461193 25461341 149 + 0.827 0.710 -0.470
ENSG00000077092 E028 2.2120043 0.082399858 8.905243e-01 0.973769065 3 25462309 25466868 4560 + 0.515 0.464 -0.251
ENSG00000077092 E029 6.6652139 0.005646823 7.808774e-01 0.942493537 3 25501182 25501323 142 + 0.866 0.884 0.069
ENSG00000077092 E030 0.0000000       3 25534680 25534715 36 +      
ENSG00000077092 E031 0.0000000       3 25535436 25535464 29 +      
ENSG00000077092 E032 6.9417841 0.003618510 4.387233e-01 0.796919615 3 25569758 25569904 147 + 0.950 0.847 -0.394
ENSG00000077092 E033 4.5287548 0.005635946 4.090113e-01 0.779689616 3 25569905 25569918 14 + 0.805 0.682 -0.501
ENSG00000077092 E034 9.1589584 0.004157158 1.485703e-01 0.537108213 3 25580546 25580722 177 + 0.935 1.071 0.503
ENSG00000077092 E035 0.1717682 0.035827150 4.636272e-01   3 25582503 25582554 52 + 0.000 0.120 11.659
ENSG00000077092 E036 7.6781368 0.016792317 3.423710e-02 0.294746553 3 25593503 25593640 138 + 0.786 1.033 0.940
ENSG00000077092 E037 6.1254306 0.004700261 4.370524e-01 0.795820556 3 25593641 25593707 67 + 0.805 0.884 0.306
ENSG00000077092 E038 12.0347167 0.002642435 8.001352e-03 0.156318452 3 25594520 25594678 159 + 0.980 1.210 0.830
ENSG00000077092 E039 50.3359791 0.002706566 5.092178e-01 0.834349876 3 25596420 25597932 1513 + 1.708 1.716 0.026