ENSG00000061455

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000407847 ENSG00000061455 HEK293_DMSO_6hA HEK293_OSMI2_6hA PRDM6 protein_coding protein_coding 3.157056 2.355229 3.888372 0.1544883 0.6393977 0.7208917 0.7763366 0.6257589 0.6291441 0.15452913 0.1041713 0.007661517 0.25072917 0.2607000 0.1619 -0.09880000 0.58018688 0.02936821 FALSE TRUE
ENST00000434521 ENSG00000061455 HEK293_DMSO_6hA HEK293_OSMI2_6hA PRDM6 protein_coding nonsense_mediated_decay 3.157056 2.355229 3.888372 0.1544883 0.6393977 0.7208917 0.1445601 0.0000000 0.2679058 0.00000000 0.0755134 4.796523856 0.04347917 0.0000000 0.0748 0.07480000 0.02936821 0.02936821 FALSE FALSE
MSTRG.26820.1 ENSG00000061455 HEK293_DMSO_6hA HEK293_OSMI2_6hA PRDM6 protein_coding   3.157056 2.355229 3.888372 0.1544883 0.6393977 0.7208917 2.0756169 1.6191128 2.9554792 0.08161024 0.5566565 0.864178752 0.65304167 0.6901667 0.7538 0.06363333 0.74458911 0.02936821 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000061455 E001 0.1125166 0.0323873852 0.6077492396   5 123075746 123075803 58 + 0.101 0.001 -7.650
ENSG00000061455 E002 2.0420541 0.0098175373 0.1286823499 0.50815775 5 123089205 123089240 36 + 0.561 0.331 -1.204
ENSG00000061455 E003 2.2333299 0.0087619370 0.0197053420 0.23417022 5 123089241 123089250 10 + 0.620 0.246 -2.053
ENSG00000061455 E004 8.8399798 0.0031619092 0.2372783256 0.64232290 5 123089251 123089448 198 + 1.021 0.936 -0.314
ENSG00000061455 E005 9.0846472 0.0027767791 0.3882828318 0.76600884 5 123089449 123089519 71 + 1.020 0.973 -0.176
ENSG00000061455 E006 18.0351343 0.0142025573 0.0177746605 0.22494434 5 123090000 123090606 607 + 1.358 1.169 -0.664
ENSG00000061455 E007 9.4313826 0.0598878019 0.2575730885 0.66329768 5 123099654 123099744 91 + 1.082 0.934 -0.544
ENSG00000061455 E008 5.4776832 0.0181815368 0.4188055916 0.78560418 5 123099745 123099745 1 + 0.847 0.775 -0.285
ENSG00000061455 E009 19.0865114 0.0090181281 0.3225292416 0.71883361 5 123099746 123099961 216 + 1.325 1.285 -0.141
ENSG00000061455 E010 17.3523513 0.0015309055 0.5925978061 0.87248261 5 123155884 123156011 128 + 1.264 1.268 0.014
ENSG00000061455 E011 16.6210031 0.0019049212 0.2288039641 0.63402803 5 123159514 123159638 125 + 1.264 1.211 -0.188
ENSG00000061455 E012 38.2517625 0.0008956755 0.1652152327 0.56083404 5 123170766 123171108 343 + 1.607 1.580 -0.094
ENSG00000061455 E013 0.0000000       5 123173401 123173483 83 +      
ENSG00000061455 E014 35.8876185 0.0008755552 0.4776163120 0.81809510 5 123180147 123180323 177 + 1.563 1.571 0.028
ENSG00000061455 E015 48.1806268 0.0127628552 0.3932166116 0.76893926 5 123187087 123187332 246 + 1.689 1.681 -0.026
ENSG00000061455 E016 79.3343114 0.0052663386 0.7365757204 0.92795029 5 123187333 123187924 592 + 1.885 1.918 0.108
ENSG00000061455 E017 14.8455084 0.0020422579 0.4781956536 0.81836354 5 123187925 123188180 256 + 1.204 1.190 -0.052
ENSG00000061455 E018 102.0513820 0.0265050265 0.0009810789 0.05340788 5 123188181 123194266 6086 + 1.862 2.149 0.962