ENSG00000055332

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000233057 ENSG00000055332 HEK293_DMSO_6hA HEK293_OSMI2_6hA EIF2AK2 protein_coding protein_coding 9.00205 2.686042 4.620066 0.8720605 1.253839 0.7801898 2.6510779 0.8284497 0.9798005 0.4606036 0.1980151 0.2394136 0.28002917 0.2669000 0.27763333 0.01073333 9.762724e-01 7.629295e-10 FALSE TRUE
ENST00000405334 ENSG00000055332 HEK293_DMSO_6hA HEK293_OSMI2_6hA EIF2AK2 protein_coding protein_coding 9.00205 2.686042 4.620066 0.8720605 1.253839 0.7801898 0.9225157 0.0000000 0.6438807 0.0000000 0.6438807 6.0309556 0.10390417 0.0000000 0.09676667 0.09676667 7.597046e-01 7.629295e-10 FALSE TRUE
ENST00000647926 ENSG00000055332 HEK293_DMSO_6hA HEK293_OSMI2_6hA EIF2AK2 protein_coding nonsense_mediated_decay 9.00205 2.686042 4.620066 0.8720605 1.253839 0.7801898 0.9799177 0.0000000 1.1408120 0.0000000 0.4004545 6.8465084 0.10684167 0.0000000 0.23400000 0.23400000 8.787667e-07 7.629295e-10 TRUE TRUE
ENST00000679507 ENSG00000055332 HEK293_DMSO_6hA HEK293_OSMI2_6hA EIF2AK2 protein_coding protein_coding 9.00205 2.686042 4.620066 0.8720605 1.253839 0.7801898 0.2794158 0.2916368 0.2148433 0.2916368 0.2148433 -0.4238923 0.03968333 0.1574000 0.03230000 -0.12510000 9.981680e-01 7.629295e-10 FALSE TRUE
ENST00000681329 ENSG00000055332 HEK293_DMSO_6hA HEK293_OSMI2_6hA EIF2AK2 protein_coding processed_transcript 9.00205 2.686042 4.620066 0.8720605 1.253839 0.7801898 0.4458023 0.5404566 0.0000000 0.2885365 0.0000000 -5.7825569 0.06725000 0.2329000 0.00000000 -0.23290000 2.416340e-01 7.629295e-10 FALSE TRUE
ENST00000681507 ENSG00000055332 HEK293_DMSO_6hA HEK293_OSMI2_6hA EIF2AK2 protein_coding protein_coding 9.00205 2.686042 4.620066 0.8720605 1.253839 0.7801898 0.6846257 0.6706839 0.0000000 0.3050105 0.0000000 -6.0889132 0.07989583 0.2350333 0.00000000 -0.23503333 1.453785e-05 7.629295e-10 FALSE TRUE
MSTRG.18218.12 ENSG00000055332 HEK293_DMSO_6hA HEK293_OSMI2_6hA EIF2AK2 protein_coding   9.00205 2.686042 4.620066 0.8720605 1.253839 0.7801898 2.0193054 0.0000000 1.1823328 0.0000000 0.3260134 6.8976432 0.20230417 0.0000000 0.25353333 0.25353333 7.629295e-10 7.629295e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_6hA HEK293_DMSO_6hA log2fold_HEK293_DMSO_6hA_HEK293_OSMI2_6hA
ENSG00000055332 E001 272.9918430 0.034419993 1.887394e-06 0.0009336063 2 37099210 37101934 2725 - 2.143 2.620 1.591
ENSG00000055332 E002 20.6705016 0.027155687 3.020585e-02 0.2803978455 2 37101935 37102232 298 - 1.124 1.485 1.262
ENSG00000055332 E003 63.8521235 0.023928926 2.543067e-04 0.0238084227 2 37102233 37104014 1782 - 1.543 1.985 1.496
ENSG00000055332 E004 3.2501034 0.037821292 2.057299e-02 0.2385082995 2 37104015 37104132 118 - 0.333 0.835 2.342
ENSG00000055332 E005 27.9443260 0.014913225 4.301571e-04 0.0329546447 2 37104133 37105142 1010 - 1.185 1.641 1.579
ENSG00000055332 E006 89.4341328 0.010461044 1.327478e-01 0.5143207906 2 37105143 37107257 2115 - 1.836 2.056 0.738
ENSG00000055332 E007 17.9367970 0.001691538 1.289485e-01 0.5086035314 2 37107258 37107272 15 - 1.278 1.279 0.001
ENSG00000055332 E008 28.4026810 0.012428090 7.366223e-02 0.4034671337 2 37107273 37107345 73 - 1.479 1.456 -0.081
ENSG00000055332 E009 24.1735500 0.006279613 7.285483e-03 0.1501953333 2 37107346 37107395 50 - 1.442 1.357 -0.294
ENSG00000055332 E010 21.9843358 0.001415119 2.882857e-04 0.0257528426 2 37107474 37107527 54 - 1.433 1.294 -0.481
ENSG00000055332 E011 20.7767787 0.001516437 4.457583e-04 0.0336083927 2 37109194 37109223 30 - 1.409 1.271 -0.481
ENSG00000055332 E012 28.8177906 0.001141511 5.317963e-04 0.0373721055 2 37109224 37109295 72 - 1.532 1.436 -0.328
ENSG00000055332 E013 1.1619485 0.064778314 6.264624e-01   2 37109296 37109500 205 - 0.336 0.309 -0.171
ENSG00000055332 E014 36.6405852 0.001269650 4.505862e-04 0.0338468665 2 37114731 37114859 129 - 1.624 1.547 -0.262
ENSG00000055332 E015 22.3599319 0.001386133 1.407009e-02 0.2031672821 2 37119959 37119986 28 - 1.404 1.351 -0.181
ENSG00000055332 E016 45.4704799 0.005960187 5.974309e-05 0.0100100792 2 37119987 37120139 153 - 1.736 1.609 -0.429
ENSG00000055332 E017 39.0632114 0.011066889 4.056046e-04 0.0318813524 2 37122506 37122664 159 - 1.675 1.536 -0.474
ENSG00000055332 E018 34.5252351 0.007816551 6.032375e-03 0.1378024254 2 37126289 37126411 123 - 1.589 1.519 -0.238
ENSG00000055332 E019 30.9262479 0.001076410 1.760948e-03 0.0746205866 2 37135484 37135546 63 - 1.546 1.478 -0.233
ENSG00000055332 E020 0.0000000       2 37136729 37136982 254 -      
ENSG00000055332 E021 26.8380094 0.001130450 1.507066e-04 0.0172307268 2 37136983 37137017 35 - 1.509 1.384 -0.433
ENSG00000055332 E022 34.2689536 0.002588428 9.613574e-05 0.0132313599 2 37138270 37138363 94 - 1.611 1.493 -0.405
ENSG00000055332 E023 31.1286825 0.013296738 8.896015e-03 0.1642967769 2 37138509 37138585 77 - 1.567 1.462 -0.361
ENSG00000055332 E024 31.6768402 0.005851956 5.717752e-03 0.1347579331 2 37139631 37139757 127 - 1.562 1.487 -0.257
ENSG00000055332 E025 25.2186657 0.001463801 2.738208e-05 0.0059657876 2 37141553 37141701 149 - 1.501 1.338 -0.564
ENSG00000055332 E026 22.7730957 0.005605598 7.408683e-03 0.1516035295 2 37146853 37146973 121 - 1.433 1.344 -0.309
ENSG00000055332 E027 24.8691374 0.003896205 2.238425e-02 0.2473256660 2 37147688 37147822 135 - 1.448 1.407 -0.143
ENSG00000055332 E028 14.7588933 0.008821953 1.974250e-01 0.6006076524 2 37148857 37148915 59 - 1.205 1.209 0.015
ENSG00000055332 E029 11.3378987 0.003013012 2.435496e-01 0.6487023184 2 37148916 37148939 24 - 1.100 1.106 0.022
ENSG00000055332 E030 7.0633577 0.028742502 4.164874e-01 0.7844441093 2 37148940 37149023 84 - 0.902 0.916 0.053
ENSG00000055332 E031 1.8484488 0.227842884 6.970694e-01 0.9139659543 2 37149024 37149257 234 - 0.449 0.437 -0.062
ENSG00000055332 E032 8.4531444 0.003215626 3.976245e-03 0.1128832494 2 37156908 37157192 285 - 1.079 0.879 -0.742
ENSG00000055332 E033 0.6429549 0.021140536 5.151827e-02   2 37157367 37157561 195 - 0.000 0.377 14.181