ENSG00000196873

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000469687 ENSG00000196873 HEK293_DMSO_2hA HEK293_OSMI2_2hA CBWD3 protein_coding retained_intron 11.86615 4.695829 5.302169 0.6270875 0.1561853 0.1748521 0.8100576 0.1534780 0.22002182 0.01711435 0.02323813 0.49267421 0.06566250 0.03413333 0.041300000 0.007166667 0.97493913 0.04331455   FALSE
ENST00000478048 ENSG00000196873 HEK293_DMSO_2hA HEK293_OSMI2_2hA CBWD3 protein_coding protein_coding 11.86615 4.695829 5.302169 0.6270875 0.1561853 0.1748521 1.9843839 1.6363712 1.72353375 0.40652373 0.16653588 0.07442625 0.19754167 0.33830000 0.324366667 -0.013933333 0.97928063 0.04331455 FALSE TRUE
ENST00000615061 ENSG00000196873 HEK293_DMSO_2hA HEK293_OSMI2_2hA CBWD3 protein_coding nonsense_mediated_decay 11.86615 4.695829 5.302169 0.6270875 0.1561853 0.1748521 0.0512630 0.0000000 0.41010400 0.00000000 0.41010400 5.39267461 0.00932500 0.00000000 0.074600000 0.074600000 0.93679467 0.04331455 FALSE TRUE
ENST00000616550 ENSG00000196873 HEK293_DMSO_2hA HEK293_OSMI2_2hA CBWD3 protein_coding protein_coding 11.86615 4.695829 5.302169 0.6270875 0.1561853 0.1748521 5.4001892 0.0000000 0.49799581 0.00000000 0.49799581 5.66674470 0.35708333 0.00000000 0.091966667 0.091966667 0.93297050 0.04331455 FALSE TRUE
ENST00000619322 ENSG00000196873 HEK293_DMSO_2hA HEK293_OSMI2_2hA CBWD3 protein_coding retained_intron 11.86615 4.695829 5.302169 0.6270875 0.1561853 0.1748521 0.2776915 0.2627217 0.02304125 0.05074479 0.01245979 -3.04508937 0.02728750 0.05493333 0.004233333 -0.050700000 0.04331455 0.04331455 FALSE TRUE
MSTRG.32829.12 ENSG00000196873 HEK293_DMSO_2hA HEK293_OSMI2_2hA CBWD3 protein_coding   11.86615 4.695829 5.302169 0.6270875 0.1561853 0.1748521 0.2049801 0.2620068 0.06998547 0.13109668 0.06998547 -1.76583299 0.01948333 0.06366667 0.012733333 -0.050933333 0.92295727 0.04331455 TRUE TRUE
MSTRG.32829.13 ENSG00000196873 HEK293_DMSO_2hA HEK293_OSMI2_2hA CBWD3 protein_coding   11.86615 4.695829 5.302169 0.6270875 0.1561853 0.1748521 0.2376390 0.6553090 0.16660180 0.26511774 0.09928897 -1.91352460 0.03252500 0.12926667 0.032400000 -0.096866667 0.65949845 0.04331455 TRUE TRUE
MSTRG.32829.20 ENSG00000196873 HEK293_DMSO_2hA HEK293_OSMI2_2hA CBWD3 protein_coding   11.86615 4.695829 5.302169 0.6270875 0.1561853 0.1748521 0.5834046 0.4759081 0.79110989 0.17495401 0.12901895 0.72131681 0.06369167 0.11736667 0.150000000 0.032633333 0.92295727 0.04331455 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_OSMI2_2hA HEK293_DMSO_2hA log2fold_HEK293_DMSO_2hA_HEK293_OSMI2_2hA
ENSG00000196873 E001 1.0262252 0.016256026 1.044865e-01   9 68232003 68232198 196 + 0.174 0.436 1.808
ENSG00000196873 E002 0.9928396 0.015637592 4.209397e-01   9 68232437 68232495 59 + 0.241 0.361 0.806
ENSG00000196873 E003 0.8913382 0.016346839 1.000000e+00   9 68235000 68235128 129 + 0.298 0.270 -0.193
ENSG00000196873 E004 0.0000000       9 68241854 68241888 35 +      
ENSG00000196873 E005 0.0000000       9 68241889 68241912 24 +      
ENSG00000196873 E006 0.0000000       9 68241913 68241924 12 +      
ENSG00000196873 E007 0.0000000       9 68241925 68241925 1 +      
ENSG00000196873 E008 0.0000000       9 68241926 68241944 19 +      
ENSG00000196873 E009 0.0000000       9 68241945 68241948 4 +      
ENSG00000196873 E010 0.0000000       9 68241949 68241950 2 +      
ENSG00000196873 E011 0.0000000       9 68241951 68241953 3 +      
ENSG00000196873 E012 0.0000000       9 68241954 68241955 2 +      
ENSG00000196873 E013 0.2623788 0.030497223 5.276888e-01   9 68241956 68241982 27 + 0.174 0.000 -13.896
ENSG00000196873 E014 0.2623788 0.030497223 5.276888e-01   9 68241983 68241984 2 + 0.174 0.000 -13.896
ENSG00000196873 E015 0.2623788 0.030497223 5.276888e-01   9 68241985 68241988 4 + 0.174 0.000 -13.896
ENSG00000196873 E016 0.6147368 0.021171292 9.897508e-02   9 68241989 68242185 197 + 0.349 0.000 -14.992
ENSG00000196873 E017 0.0000000       9 68243183 68243280 98 +      
ENSG00000196873 E018 0.4279916 0.152772560 1.000000e+00   9 68245213 68245299 87 + 0.172 0.156 -0.170
ENSG00000196873 E019 0.5389803 0.021105685 2.450128e-01   9 68247613 68247709 97 + 0.096 0.270 1.805
ENSG00000196873 E020 0.8921135 0.148235615 4.747825e-01   9 68247710 68248805 1096 + 0.351 0.156 -1.525
ENSG00000196873 E021 3.1953408 0.006737220 2.751466e-02 0.93871829 9 68248806 68248897 92 + 0.756 0.361 -1.860
ENSG00000196873 E022 0.1088301 0.056760012 1.000000e+00   9 68248898 68249031 134 + 0.096 0.000 -13.055
ENSG00000196873 E023 0.0000000       9 68253356 68254360 1005 +      
ENSG00000196873 E024 0.0000000       9 68254361 68254977 617 +      
ENSG00000196873 E025 3.5735665 0.005819240 9.966910e-03 0.73552266 9 68256921 68256980 60 + 0.809 0.361 -2.071
ENSG00000196873 E026 1.5930418 0.010984272 2.460278e-01   9 68258532 68258569 38 + 0.508 0.270 -1.365
ENSG00000196873 E027 1.7086587 0.010588793 3.778739e-02   9 68259347 68259434 88 + 0.571 0.156 -2.656
ENSG00000196873 E028 0.2103315 0.035360146 2.351390e-01   9 68260857 68260996 140 + 0.000 0.156 13.802
ENSG00000196873 E029 0.0000000       9 68260997 68261001 5 +      
ENSG00000196873 E030 3.2097526 0.006986639 9.233721e-01 0.99995031 9 68264468 68264514 47 + 0.625 0.605 -0.090
ENSG00000196873 E031 3.5279508 0.005899388 1.533315e-02 0.83556550 9 68266220 68266306 87 + 0.474 0.791 1.389
ENSG00000196873 E032 2.8776699 0.008123795 1.878886e-02 0.87704283 9 68268934 68268978 45 + 0.395 0.726 1.541
ENSG00000196873 E033 0.1346978 0.062841107 1.000000e+00   9 68268979 68269599 621 + 0.096 0.000 -13.059
ENSG00000196873 E034 2.7406908 0.011167401 5.980675e-05 0.08776613 9 68269600 68269722 123 + 0.174 0.791 3.391
ENSG00000196873 E035 0.0000000       9 68269723 68269851 129 +      
ENSG00000196873 E036 0.4206629 0.075639232 7.681078e-02   9 68269852 68269896 45 + 0.000 0.271 14.670
ENSG00000196873 E037 0.0000000       9 68269897 68270301 405 +      
ENSG00000196873 E038 0.2693956 0.480706287 6.877297e-01   9 68270302 68270385 84 + 0.176 0.000 -13.856
ENSG00000196873 E039 0.1346978 0.062841107 1.000000e+00   9 68270386 68270390 5 + 0.096 0.000 -13.059
ENSG00000196873 E040 0.1346978 0.062841107 1.000000e+00   9 68270391 68270518 128 + 0.096 0.000 -13.059
ENSG00000196873 E041 0.0000000       9 68270519 68270559 41 +      
ENSG00000196873 E042 0.4374789 0.023974329 1.000000e+00   9 68270560 68270895 336 + 0.174 0.156 -0.194
ENSG00000196873 E043 0.4821975 0.023570324 1.000000e+00   9 68272406 68272991 586 + 0.175 0.156 -0.198
ENSG00000196873 E044 0.2882465 0.030489925 5.267877e-01   9 68272992 68273003 12 + 0.175 0.000 -13.899
ENSG00000196873 E045 0.2882465 0.030489925 5.267877e-01   9 68273004 68273035 32 + 0.175 0.000 -13.899
ENSG00000196873 E046 0.3970766 0.024430794 2.862814e-01   9 68273036 68273197 162 + 0.241 0.000 -14.378
ENSG00000196873 E047 1.5968302 0.153838756 6.847870e-01   9 68273198 68274119 922 + 0.397 0.434 0.200
ENSG00000196873 E048 0.7148556 0.222623472 4.745316e-01   9 68274120 68274881 762 + 0.178 0.270 0.771
ENSG00000196873 E049 1.2884148 0.012710294 4.476443e-01   9 68274882 68274938 57 + 0.436 0.270 -1.001
ENSG00000196873 E050 0.6055622 0.267289293 5.644897e-01   9 68285330 68285988 659 + 0.171 0.269 0.830
ENSG00000196873 E051 0.6217535 0.111270597 1.241669e-01   9 68285989 68286040 52 + 0.348 0.000 -14.968
ENSG00000196873 E052 2.0625237 0.009524106 7.252502e-01 0.99995031 9 68297580 68297648 69 + 0.474 0.499 0.126
ENSG00000196873 E053 2.0625237 0.009524106 7.252502e-01 0.99995031 9 68297752 68297817 66 + 0.474 0.499 0.126
ENSG00000196873 E054 0.1939510 0.037382240 2.349650e-01   9 68297818 68298963 1146 + 0.000 0.156 13.800
ENSG00000196873 E055 2.1880461 0.193391093 8.214576e-01 0.99995031 9 68298964 68299093 130 + 0.565 0.435 -0.633
ENSG00000196873 E056 0.5651599 0.023877156 7.753209e-01   9 68299094 68299480 387 + 0.241 0.156 -0.778
ENSG00000196873 E057 0.8606123 0.016167867 2.353967e-01   9 68299481 68300035 555 + 0.174 0.361 1.390